Intercellular trafficking and protein delivery by a herpesvirus structural protein
Intercellular trafficking and protein delivery by a herpesvirus structural protein
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DOI：
10.1016/s0092-8674(00)81843-7
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发表时间：
1997-01-24
期刊：
影响因子：
64.5
通讯作者：
OHare, P
中科院分区：
文献类型：
--
作者：
Elliott, G;OHare, P
我们发现HSV-1结构蛋白VP22具有显著的细胞间运输特性，这种特性非常有效，以至于在亚群中表达后，该蛋白以单层形式扩散到每个细胞，在那里它集中在细胞核中并结合染色质。在DNA转染或显微注射后以及病毒感染过程中均观察到VP22的运动。此外，我们证明VP22的转运是通过非经典的高尔基无关机制发生的。对细胞松弛素D治疗的敏感性表明VP22利用了一种涉及肌动蛋白细胞骨架的新型运输途径。此外，我们证实了VP22融合蛋白在内源性合成或外源性应用后的细胞间转运，这表明VP22可能在蛋白质递送领域具有潜力。
We show that the HSV-1 structural protein VP22 has the remarkable property of intercellular transport, which is so efficient that following expression in a subpopulation the protein spreads to every cell in a monolayer, where it concentrates in the nucleus and binds chromatin. VP22 movement was observed both after delivery of DNA by transfection or microinjection and during virus infection. Moreover, we demonstrate that VP22 trafficking occurs via a nonclassical Golgi-independent mechanism. Sensitivity to cytochalasin D treatment suggests that VP22 utilizes a novel trafficking pathway that involves the actin cytoskeleton. In addition, we demonstrate intercellular transport of a VP22 fusion protein after endogenous synthesis or exogenous application, indicating that VP22 may have potential in the field of protein delivery.