Genotyping with TaqMAMA
Genotyping with TaqMAMA
复制标题
DOI：
10.1016/j.ygeno.2003.08.005
复制
发表时间：
2004-02-01
期刊：
影响因子：
4.4
通讯作者：
Watson, DE
中科院分区：
文献类型：
--
作者：
Li, BH;Kadura, I;Watson, DE
TaqMAMA结合了TaqMan的定量优势和MAMA的等位基因特异性PCR。在这篇文章中，我们开发了TaqMAMA作为一种技术，用于筛选已知遗传多态性的人类DNA样本。在第一组实验中，使用模拟所有类型的遗传多态性的质粒来了解TaqMAMA引物/模板错配与其等位基因区分强度之间的关系。这些数据可用于通过直接使用核苷酸错配来改善其他引物延伸基因分型方法的等位基因区分。我们使用这些数据来获得TaqMAMA引物设计和TaqMAMA基因分型测定的DNA链选择的指导。然后，该指南被用于开发11种已知和新的人类遗传多态性的检测方法。在所有情况下，基因型被快速准确地分配。TaqMAMA基因分型检测需要最少的开发时间，具有很高的成功概率，产生可靠的数据，易于分析，并且具有很强的成本竞争力。(C)2003年爱思唯尔公司All rights reserved.
TaqMAMA combines the quantitative strengths of TaqMan with the allele-specific PCR of MAMA. In this article we develop TaqMAMA as a technique for screening human DNA samples for known genetic polymorphisms. In the first set of experiments, plasmids that model all types of genetic polymorphisms were used to understand the relationship between TaqMAMA primer/template mismatches and their strength of allelic discrimination. These data can be used to improve allelic discrimination of other primer extension genotyping methodologies through directed use of nucleotide mismatches. We used the data to derive a guide for TaqMAMA primer design and DNA strand selection for TaqMAMA genotyping assays. The guide was then used to develop assays for 11 known and novel human genetic polymorphisms. Genotypes were assigned quickly and accurately in all cases. TaqMAMA genotyping assays require minimal development time, have a high probability of success, produce reliable data that are straightforward to analyze, and are very cost-competitive. (C) 2003 Elsevier Inc. All rights reserved.