High-Resolution Structural Proteomics of Mitochondria Using the 'Build and Retrieve' Methodology.
High-Resolution Structural Proteomics of Mitochondria Using the 'Build and Retrieve' Methodology.
复制标题
DOI：
10.1016/j.mcpro.2023.100666
复制
发表时间：
2023-12
影响因子：
7
通讯作者：
Yu, Edward W.
中科院分区：
文献类型：
--
作者：
Zhang, Zhemin;Tringides, Marios L.;Morgan, Christopher E.;Miyagi, Masaru;Mears, Jason A.;Hoppel, Charles L.;Yu, Edward W.
整合系统生物学在结构生物学领域的应用是一个有前途的新方向，尽管它仍处于发展的婴儿阶段。在这里，我们报告使用单颗粒cryo-EM，以确定多种蛋白质从三个丰富的异质馏分制备的人肝线粒体裂解液。我们同时确定和解决9个关键代谢功能，包括脂肪酸催化剂，活性氧化物质清除和氨基酸代谢的基本线粒体酶的高分辨率结构。我们的方法还鉴定了酰基辅酶A脱氢酶家族的多个不同成员。这项工作突出了cryo-EM在原子水平上探索组织蛋白质组学的潜力。使用冷冻EM来确定人线粒体裂解物的组成。同时识别和解决9种线粒体酶的结构。cryo-EM在人类酶高分辨率结构组学研究中的应用Zhang等人使用cryo-EM从人肝线粒体裂解物中同时鉴定和解析9种不同酶的结构。他们的工作突出了这种方法在促进高分辨率生物系统的结构蛋白质组学方面的潜力。
The application of integrated systems biology to the field of structural biology is a promising new direction, although it is still in the infant stages of development. Here we report the use of single particle cryo-EM to identify multiple proteins from three enriched heterogeneous fractions prepared from human liver mitochondrial lysate. We simultaneously identify and solve high-resolution structures of nine essential mitochondrial enzymes with key metabolic functions, including fatty acid catabolism, reactive oxidative species clearance, and amino acid metabolism. Our methodology also identified multiple distinct members of the acyl-CoA dehydrogenase family. This work highlights the potential of cryo-EM to explore tissue proteomics at the atomic level. Using cryo-EM to define the composition of a human mitochondrial lysate. Simultaneously identifying and solving structures of nine mitochondrial enzymes. Application of cryo-EM in studying high-resolution structural omics of human enzymes. Zhang et al use cryo-EM to simultaneously identify and solve structures of nine different enzymes from human liver mitochondrial lysate. Their work highlights the potential of this methodology in facilitating structural proteomics of a biological system at high resolution.
登录
查看更多内容
影响因子：
3.5
作者：
Maier EM;Gersting SW;Kemter KF;Jank JM;Reindl M;Messing DD;Truger MS;Sommerhoff CP;Muntau AC
通讯作者：
Muntau AC
DOI：
10.1073/pnas.90.16.7523
发表时间：
1993-08-15
影响因子：
11.1
作者：
KIM, JJP;WANG, M;PASCHKE, R
通讯作者：
PASCHKE, R
影响因子：
64.5
作者：
BORGSTAHL, GEO;PARGE, HE;TAINER, JA
通讯作者：
TAINER, JA
影响因子：
2.9
作者：
Banerjee, S;Schmidt, T;Smith, TJ
通讯作者：
Smith, TJ
影响因子：
4.5
作者：
McInnes J
通讯作者：
McInnes J