Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells
Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells
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DOI：
10.1073/pnas.93.1.316
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发表时间：
1996-01-09
影响因子：
11.1
通讯作者：
Rochefort, H
中科院分区：
文献类型：
--
作者：
Clinton, GM;Rougeot, C;Rochefort, H
卵巢癌侵袭腹腔的能力很强，其中一些是由雌激素刺激的，为了了解雌激素对这些癌细胞的作用模式并开发新的标志物，我们对雌激素调节蛋白进行了表征，本研究旨在鉴定雌二醇增加卵巢癌细胞分泌的一种蛋白[Galtier-Dereure， F., Capony， F., Maudelonde， T. & Rochefort， H. (1992) J. Clin]。性。中国生物医学工程学报，2004,18(2):444 - 444。通过微蛋白测序，110 kda蛋白被鉴定为纤维蛋白-1，这是一种细胞外基质蛋白，与纤维连接蛋白、层粘连蛋白和氮素结合。在三种雌激素反应性卵巢癌细胞系中，经雌二醇处理后，分泌到培养基和细胞提取物中的免疫沉淀纤维蛋白-1的量增加了10倍。免疫组化发现纤维蛋白-1存在于几种卵巢癌和囊肿的基质中。这些发现强调了纤维蛋白-1在卵巢癌腹腔扩散和/或远端转移中的潜在作用。
Ovarian cancers have a high ability to invade the peritoneal cavity and some are stimulated by estrogens, In an attempt to understand the mode of action of estrogens on these cancer cells and to develop new markers, we have characterized estrogen-regulated proteins, This study,vas aimed at identifying a protein secreted by ovarian cancer cells whose level was increased by estradiol [Galtier-Dereure, F., Capony, F., Maudelonde, T. & Rochefort, H. (1992) J. Clin. Endocrinol. Metab, 75, 1197-1502]. By using microprotein sequencing, the 110-kDa protein was identified as fibulin-1, a protein of the extracellular matrix that binds to fibronectin, laminin, and nidogen, The amount of immunoprecipitated fibulin-1 secreted into the medium and present in the cell extract was increased up to 10-fold by estradiol in three estrogen-responsive ovarian cancer cell lines. By immunohistochemistry fibulin-1 was located in the stroma of several ovarian cancers and cysts. The findings highlight a potential role for fibulin-1 in the spread of ovarian cancer in the peritoneal cavity and/or in distal metastases.