Enterococcal Linear Plasmids Adapt to Enterococcus faecium and Spread within Multidrug-Resistant Clades
Enterococcal Linear Plasmids Adapt to Enterococcus faecium and Spread within Multidrug-Resistant Clades
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DOI：
10.1128/aac.01619-22
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发表时间：
2023-03-28
影响因子：
4.9
通讯作者：
Tomita, Haruyoshi
中科院分区：
文献类型：
--
作者：
Hashimoto, Yusuke;Suzuki, Masato;Tomita, Haruyoshi
包括肠球菌在内的细菌性病原体的抗微生物药物耐药性（AMR）是一个全球关注的问题，质粒对于AMR基因的传播和维持至关重要。近年来在临床多药耐药肠球菌中发现了具有线性拓扑结构的质粒。肠球菌线状质粒，如pELF1，赋予对临床重要抗菌素（包括万古霉素）的耐药性；然而，关于其流行病学和生理学作用的资料很少。在这项研究中，我们确定了肠球菌线性质粒的几个谱系，它们在结构上是保守的，并且在全球范围内存在。类似pelf1的线性质粒在获取和维持AMR基因方面表现出可塑性，通常通过与可移动遗传元件IS1216E的转座。这个线性质粒家族具有几个特点，可以在细菌群体中长期存在，包括高水平自传性，质粒携带基因的低水平转录，以及对粪肠球菌基因组的适度影响，减轻了适应成本，促进了垂直遗传。综合所有这些因素，线性质粒是AMR基因在肠球菌中传播和维持的重要因素。包括肠球菌在内的细菌性病原体的抗微生物药物耐药性（AMR）是一个全球关注的问题，质粒对于AMR基因的传播和维持至关重要。近年来在临床多药耐药肠球菌中发现了具有线性拓扑结构的质粒。
Antimicrobial resistance (AMR) of bacterial pathogens, including enterococci, is a global concern, and plasmids are crucial for spreading and maintaining AMR genes. Plasmids with linear topology were identified recently in clinical multidrug-resistant enterococci. The enterococcal linear-form plasmids, such as pELF1, confer resistance to clinically important antimicrobials, including vancomycin; however, little information exists about their epidemiological and physiological effects. In this study, we identified several lineages of enterococcal linear plasmids that are structurally conserved and occur globally. pELF1-like linear plasmids show plasticity in acquiring and maintaining AMR genes, often via transposition with the mobile genetic element IS1216E. This linear plasmid family has several characteristics enabling long-term persistence in the bacterial population, including high horizontal self-transmissibility, low-level transcription of plasmid-carried genes, and a moderate effect on the Enterococcus faecium genome alleviating fitness cost and promoting vertical inheritance. Combining all of these factors, the linear plasmid is an important factor in the spread and maintenance of AMR genes among enterococci.Antimicrobial resistance (AMR) of bacterial pathogens, including enterococci, is a global concern, and plasmids are crucial for spreading and maintaining AMR genes. Plasmids with linear topology were identified recently in clinical multidrug-resistant enterococci.