ATF3 suppresses ESCC via downregulation of ID1.
ATF3 suppresses ESCC via downregulation of ID1.
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DOI：
10.3892/ol.2016.4832
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发表时间：
2016-09
期刊：
影响因子：
2.9
通讯作者：
Yang W
中科院分区：
文献类型：
--
作者：
Li J;Yang Z;Chen Z;Bao Y;Zhang H;Fang X;Yang W
食管癌是最普遍的癌症形式之一，并且由于早期转移而具有特别高的死亡率;然而，其形成和进展的潜在机制仍不清楚。本研究通过免疫组化分析，观察到转录激活因子3（ATF 3）的表达在食管鳞状细胞癌（ESCC）与非癌旁组织相比，减少。DNA结合抑制因子1（inhibitor of DNA binding 1，ID 1）在食管鳞癌组织中呈高表达，与ATF 3呈负相关（P<0.01）。在ESCC EC 109和KYSE 450细胞系中，转染ATF 3过表达质粒导致细胞增殖、运动和迁移的抑制，这与诱导E-cadherin表达和抑制cyclin D1和Twist有关。值得注意的是，ATF 3发挥了与ID 1的反向调节相互作用。本研究的结果为ATF 3的肿瘤抑制特征提供了额外的证据，并证明了ATF 3介导的抑制食管癌转移的新机制。
Esophageal cancer is one of the most prevalent forms of cancer and has a particularly high mortality rate due to early metastasis; however, the underlying mechanisms of its formation and progression remain unclear. The present study performed immunohistochemical analysis and observed that the expression of activating transcription factor 3 (ATF3) was reduced in esophageal squamous cell carcinoma (ESCC) in comparison with non-tumor adjacent tissues. By contrast, inhibitor of DNA binding 1 (ID1) was overexpressed in ESCC tissues, demonstrating an inverse correlation with ATF3 (P<0.01). In ESCC EC109 and KYSE450 cells lines, transfection with an ATF3-overexpression plasmid resulted in the inhibition of cell proliferation, motility and migration, which was associated with the induction of E-cadherin expression and inhibition of cyclin D1 and Twist. Notably, ATF3 exerted an inverse regulatory interaction with ID1. The results of the present study provide additional evidence of the tumor suppressive features of ATF3 and demonstrate a novel mechanism of ATF3-mediated inhibition of cancer metastasis in esophageal cancer.