Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
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DOI：
10.3390/ijms19123803
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发表时间：
2018-12-01
影响因子：
5.6
通讯作者：
Baba, Shunsuke
中科院分区：
文献类型：
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作者：
Sasayama, Satoshi;Hara, Tomoya;Baba, Shunsuke
基于干细胞的再生医学对具有成本效益和功能化的支架有很高的需求，以治疗颅面畸形和损伤中的难治性骨缺损。一种潜在的策略是利用药理学和经济有效的植物多酚和生物相容性蛋白质，例如明胶。然而，在该策略中使用化学修饰的蛋白质和植物多酚尚未标准化。在这里，我们证明，用表没食子儿茶素没食子酸酯（EGCG）（从绿茶中分离出的主要儿茶素）进行化学修饰的明胶，在与/不与脂肪干细胞或去分化脂肪细胞一起使用时，可以成为诱导大鼠先天性颌裂模型体内骨再生的有用材料。与真空加热明胶海绵 (vhGS) 相比，用 EGCG 修饰的真空加热明胶海绵 (vhEGCG-GS) 诱导这两种细胞类型产生更好的成骨作用。 EGCG修饰将vhGS的水润湿性转化为亲水性（接触角：110度至3.8度），并将zeta电位转化为负表面电荷；该修饰增强了细胞粘附性能并促进磷酸钙沉淀。这些结果表明，化学合成的 EGCG 修饰可以成为改变明胶理化性质的有用平台。这种改变可能为多能祖细胞提供更好的微环境，诱导体内更好的骨形成。
Cost-effective and functionalized scaffolds are in high demand for stem-cell-based regenerative medicine to treat refractory bone defects in craniofacial abnormalities and injuries. One potential strategy is to utilize pharmacological and cost-effective plant polyphenols and biocompatible proteins, such as gelatin. Nevertheless, the use of chemically modified proteins with plant polyphenols in this strategy has not been standardized. Here, we demonstrated that gelatin chemically modified with epigallocatechin gallate (EGCG), the major catechin isolated from green tea, can be a useful material to induce bone regeneration in a rat congenial cleft-jaw model in vivo when used with/without adipose-derived stem cells or dedifferentiated fat cells. Vacuum-heated gelatin sponges modified with EGCG (vhEGCG-GS) induced superior osteogenesis from these two cell types compared with vacuum-heated gelatin sponges (vhGS). The EGCG-modification converted the water wettability of vhGS to a hydrophilic property (contact angle: 110 degrees to 3.8 degrees) and the zeta potential to a negative surface charge; the modification enhanced the cell adhesion property and promoted calcium phosphate precipitation. These results suggest that the EGCG-modification with chemical synthesis can be a useful platform to modify the physicochemical property of gelatin. This alteration is likely to provide a preferable microenvironment for multipotent progenitor cells, inducing superior bone formation in vivo.