Antimicrobial properties of mucus from the brown garden snail Helix aspersa
Antimicrobial properties of mucus from the brown garden snail Helix aspersa
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DOI：
10.1080/09674845.2015.11665749
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发表时间：
2015-01-01
影响因子：
1.9
通讯作者：
Gunn, A.
中科院分区：
文献类型：
--
作者：
Pitt, S. J.;Graham, M. A.;Gunn, A.
对天然抗菌物质的研究已经产生了有效的治疗方法。一个感兴趣的领域是无脊椎动物产生的肽和蛋白质作为其防御系统的一部分，包括软体动物粘液的内容物。据报道，非洲巨型陆生蜗牛Achatina fulica产生的粘液含有两种具有广谱抗菌活性的蛋白质。来自棕色花园蜗牛的粘液似乎具有皮肤再生特性。本研究旨在探讨H. aspersa粘液。从H.用磷酸盐缓冲盐水（PBS）稀释的aspersa蜗牛，在圆盘扩散抗微生物测定中测试上清液对广泛的生物体的作用。fulica，包括噬菌体测定。使来自两种蜗牛的粘液通过一系列蛋白质大小分离柱以确定抗微生物物质的大致大小。对H. aspersa粘液。结果表明，H. aspersa粘液对几种铜绿假单胞菌有较强的抗菌作用，对金黄色葡萄球菌的抗菌作用较弱。A. fulica对S.金黄色葡萄球菌，但没有观察到其他工人报告的广谱活性。抗菌活性不是由噬菌体引起的。粒度分离实验表明，H. aspersa之间的30和100 kDa。电泳显示该区域中有两种蛋白质（30 - 40 kDa和50 - 60 kDa）。这些蛋白与以前在A.富里卡。本研究发现了一种或多种新的抗菌药物。aspersa粘液，对铜绿假单胞菌有很强的作用。
Research into naturally occurring antimicrobial substances has yielded effective treatments. One area of interest is peptides and proteins produced by invertebrates as part of their defence system, including the contents of mollusc mucus. Mucus produced by the African giant land snail, Achatina fulica has been reported to contain two proteins with broad-spectrum antibacterial activity. Mucus from the brown garden snail, Helix aspersa, appears to have skin regeneration properties. This study sought to investigate the antimicrobial properties of H. aspersa mucus. Mucus was collected from H. aspersa snails, diluted in phosphate-buffered saline (PBS), with the supernatant tested against a wide range of organisms in a disc-diffusion antimicrobial assay This was followed with comparative experiments involving A. fulica, including bacteriophage assays. Mucus from both species of snail was passed through a series of protein size separation columns in order to determine the approximate size of the antimicrobial substance. Electrophoresis was also carried out on the H. aspersa mucus. Results indicated that H. aspersa mucus had a strong antibacterial effect against several strains of Pseudomonas aeruginosa and a weak effect against Staphylococcus aureus. Mucus from A. fulica also inhibited the growth of S. aureus, but the broad spectrum of activity reported by other workers was not observed. Antimicrobial activity was not caused by bacteriophage. Size separation experiments indicated that the antimicrobial substance(s) in H. aspersa were between 30 and 100 kDa. Electrophoresis revealed two proteins in this region (30-40 kDa and 50-60 kDa). These do not correspond with antimicrobial proteins previously reported in A. fulica. This study found one or more novel antimicrobial agents in H. aspersa mucus, with a strong effect against P. aeruginosa.