RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation
RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation
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DOI：
10.1111/gtc.12562
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发表时间：
2018-03-01
期刊：
影响因子：
2.1
通讯作者：
Tani, Tokio
中科院分区：
文献类型：
--
作者：
Cho, Yukiko;Ideue, Takashi;Tani, Tokio
卫星I RNA是一种从人类着丝粒重复区域转录而来的非编码(NC)RNA，它与极光激酶B结合，形成染色体分离所需的ncRNP复合体。为了研究它在这一过程中的作用，我们从异步化或有丝分裂(M)期阻滞的HeLa细胞的核提取液中纯化了卫星I ncRNP复合体，然后进行了LC/MS鉴定与卫星I RNA结合的蛋白质。对从M期细胞中分离得到的RNA结合基序蛋白(RNAbindingMotif Protein，X-Linked)进行了进一步鉴定。我们发现，RBMX仅在M期与卫星I RNA结合。RBMX基因敲除可导致姐妹染色单体凝聚提前分离和核分裂异常。同样，卫星I RNA的敲除也导致M期姐妹染色单体过早分离。在卫星I RNA耗尽的细胞中，RBMX和凝聚力调节因子Sororin的数量减少。这些结果表明，卫星I RNA在稳定ncRNP复合体中的RBMX和Sororin以维持适当的姐妹染色单体凝聚力方面发挥了作用。
Satellite I RNA, a noncoding (nc)RNA transcribed from repetitive regions in human centromeres, binds to Aurora kinase B and forms a ncRNP complex required for chromosome segregation. To examine its function in this process, we purified satellite I ncRNP complex from nuclear extracts prepared from asynchronized or mitotic (M) phase-arrested HeLa cells and then carried out LC/MS to identify proteins bound to satellite I RNA. RBMX (RNA-binding motif protein, X-linked), which was isolated from M phase-arrested cells, was selected for further characterization. We found that RBMX associates with satellite I RNA only during M phase. Knockdown of RBMX induced premature separation of sister chromatid cohesion and abnormal nuclear division. Likewise, knockdown of satellite I RNA also caused premature separation of sister chromatids during M phase. The amounts of RBMX and Sororin, a cohesion regulator, were reduced in satellite I RNA-depleted cells. These results suggest that satellite I RNA plays a role in stabilizing RBMX and Sororin in the ncRNP complex to maintain proper sister chromatid cohesion.