Nonisotopic, semiautomated Plasmodium falciparum bioassay for measurement of antimalarial drug levels in serum or plasma
Nonisotopic, semiautomated Plasmodium falciparum bioassay for measurement of antimalarial drug levels in serum or plasma
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DOI：
10.1128/aac.48.11.4485-4487.2004
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发表时间：
2004-11-01
影响因子：
4.9
通讯作者：
Miller, RS
中科院分区：
文献类型：
--
作者：
Noedl, H;Teja-Isavadharm, P;Miller, RS
一种简单的，非同位素，半自动化的生物测定方法，用于测定血浆或血清中的抗疟药物水平，该方法基于疟疾培养物中富含组氨酸的蛋白II的定量。该方法只需要少量的样品，并且具有很高的灵敏度和可重复性。结果与同位素生物测定法（R = 0.988, P < 0.001）和高效液相色谱-电化学检测法（R = 0.978, P < 0.001）基本一致。
A simple, nonisotopic, semiautomated bioassay for the measurement of antimalarial drug levels in plasma or serum based on the quantitation of histidine-rich protein II in malaria culture is presented. The assay requires only small sample volumes and was found to be highly sensitive and reproducible. The results closely paralleled those obtained with isotopic bioassays (R = 0.988, P < 0.001) and high-performance liquid chromatography-electrochemical detection (R = 0.978, P < 0.001).