The distribution of trigeminovascular afferents in the nonhuman primate brain Macaca nemestrina: A c-fos immunocytochemical study
The distribution of trigeminovascular afferents in the nonhuman primate brain Macaca nemestrina: A c-fos immunocytochemical study
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DOI：
10.1046/j.1469-7580.1997.19030367.x
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发表时间：
1997-04-01
影响因子：
2.4
通讯作者：
Hoskin, KL
中科院分区：
文献类型：
--
作者：
Goadsby, PJ;Hoskin, KL
对偏头痛的理解必须基于关于疼痛敏感的颅内结构的解剖学和生理学的数据。刺激上级矢状窦产生脑血流量的变化和神经肽水平的变化，类似于在偏头痛期间在人类中观察到的变化。为了更好地了解疼痛敏感的颅内结构的中央分支的解剖结构，我们已经检查了c-fos免疫反应性的分布在猴子的鼻窦刺激时，六个成年猕猴nemestrina猴麻醉。中线开颅后分离上级矢状窦并制作石蜡孔。在手术完成后24小时，电刺激窦1小时，随后取出脑并处理c-fos。在对照组动物中，窦是孤立的，但没有刺激有少量的c-fos表达在尾侧脑干和上颈脊髓。刺激上级矢状窦诱发三叉神经核尾侧浅层和上颈髓C1节段背角浅层c-fos表达。在C2处观察到较少量的c-fos，而在C3处未观察到高于对照的显著标记。这些数据，而在很大程度上证实了猫的结果，关于中央分布trigeminovascular传入，强调可能是一个独特的专业化trigeminovascular传入在C1水平。考虑到猴子与人类的密切进化关系，这项研究中描述的细胞很可能代表了灵长类动物介导偏头痛疼痛的细胞核。
An understanding of migraine must be based on data concerning the anatomy and physiology of the pain-sensitive intracranial structures, Stimulation of the superior sagittal sinus produces changes in brain blood flow and changes in neuropeptide levels similar to those seen in humans during migraine. To better understand the anatomy of the central ramifications of pain-sensitive intracranial structures we have examined the distribution of c-fos immunoreactivity in the monkey when the sinus is stimulated, Six adult Macaca nemestrina monkeys were anaesthetised. The superior sagittal sinus was isolated after a midline craniotomy and a paraffin well created. At 24 h after completion of the surgery the sinus was stimulated electrically for 1 h and the brain subsequently removed and processed for c-fos. In control animals in which the sinus was isolated but not stimulated there was a small amount of c-fos expression in the caudal brainstem and upper cervical spinal cord. Stimulation of the superior sagittal sinus evoked expression of c-fos in the caudal superfical laminae of the trigeminal nucleus and in superficial laminae of the dorsal horn of the C1 level of the upper cervical spinal cord. A lesser amount of c-fos was seen at C2 while no significant labelling above control was observed at C3. These data, while largely confirming the results from the cat concerning the central distribution trigeminovascular afferents, underscore a possibly unique specialisation of trigeminovascular afferents at the C1 level. Given the close evolutionary relationship of the monkey to man it is likely that the cells described in this study represent for primates the nucleus that mediates the pain of migraine.