Single Iron Site Nanozyme for Ultrasensitive Glucose Detection
Single Iron Site Nanozyme for Ultrasensitive Glucose Detection
复制标题
用于超灵敏葡萄糖检测的单铁位点纳米酶
DOI：
10.1002/smll.202002343
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发表时间：
2020-06-29
期刊：
影响因子：
13.3
通讯作者：
Wu, Yuen
中科院分区：
文献类型：
--
作者：
Chen, Min;Zhou, Huang;Wu, Yuen
具有模拟酶特性的纳米材料由于其相对低成本、高稳定性和可大规模制备而引起了各个领域的广泛关注。然而，纳米酶的广泛应用受到催化活性相对较低和特异性差的严重限制，这主要是由于催化位点不均匀和催化机制不清楚。在此，证明了一种牺牲载体的策略来制备分散在多孔氮掺杂碳上的单铁位点纳米酶（Fe SSN）。 Fe SSN具有明确的配位结构和高密度的活性位点，通过有效地将H(2)O(2)激活为羟基自由基（中心点OH）物种，表现出显着的过氧化物酶样活性。此外，Fe SSN 通过多酶生物催化级联平台应用于葡萄糖的比色检测。此外，设计了一种低成本集成的基于琼脂糖的水凝胶比色生物传感器，并成功实现了葡萄糖的可视化评估和定量检测。这项工作扩大了单中心催化剂在基于纳米酶的生物传感器和个人生物医学诊断领域的应用。
Nanomaterials with enzyme-mimicking characteristics have engaged great awareness in various fields owing to their comparative low cost, high stability, and large-scale preparation. However, the wide application of nanozymes is seriously restricted by the relatively low catalytic activity and poor specificity, primarily because of the inhomogeneous catalytic sites and unclear catalytic mechanisms. Herein, a support-sacrificed strategy is demonstrated to prepare a single iron site nanozyme (Fe SSN) dispersed on the porous N-doped carbon. With well-defined coordination structure and high density of active sites, the Fe SSN performs prominent peroxidase-like activity by efficiently activating H(2)O(2)into hydroxyl radical (center dot OH) species. Furthermore, the Fe SSN is applied in colorimetric detection of glucose through a multienzyme biocatalytic cascade platform. Moreover, a low-cost integrated agarose-based hydrogel colorimetric biosensor is designed and successfully achieves the visualization evaluation and quantitative detection of glucose. This work expands the application of single-site catalysts in the fields of nanozyme-based biosensors and personal biomedical diagnosis.