Lactoferrin stimulates the expression of vitamin D receptor in vitamin D deficient mice
Lactoferrin stimulates the expression of vitamin D receptor in vitamin D deficient mice
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乳铁蛋白刺激维生素 D 缺乏小鼠中维生素 D 受体的表达
DOI：
10.1016/j.jff.2019.02.012
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发表时间：
2019-04-01
影响因子：
5.6
通讯作者：
Guo, Huiyuan
中科院分区：
文献类型：
--
作者：
Wang, Jingxuan;Li, Yixuan;Guo, Huiyuan
维生素D缺乏是一个日益严重的公共卫生问题，与血清促炎细胞因子升高和肠道微生物生态失调有关。乳铁蛋白（LF）是一种强阳离子糖蛋白，是乳中的多功能成分。本实验观察了LF（100 mg/kg和1000 mg/kg）对维生素D缺乏小鼠肠道的影响。结果表明，LF降低了颤杆菌的丰度，增加了毛螺菌科、粪杆菌属和乳酸杆菌的比例。此外，LF还可减轻维生素D缺乏引起的血清LBP、TNF-α、IL-6和TGF-β升高。值得注意的是，我们发现维生素D缺乏小鼠结肠中维生素D受体的表达在LF补充后显著增加。同时，LF还能降低维生素D缺乏小鼠结肠TLR-4的表达，抑制NF-κ B B P65的活性，增强肠屏障蛋白ZO-1、occludin、claudin-1和E-cadherin的表达。
Vitamin D deficiency, a growing public health problem, is associated with elevated serum pro-inflammatory cytokines and gut microbial dysbiosis. Lactoferrin (LF), a strongly cationic glycoprotein, is an multifunctional components of milk. We explored the effects of oral administratation with LF (100 mg/kg and 1000 mg/kg) on intestine of vitamin D deficient mice. Our results showed that LF reduced the abundance of Oscillibacter, increased the proportion of Lachnospiraceae, Faecalibaculum and Lactobacillus. In addition, LF alleviated the elevated serum LBP, TNF-alpha, IL-6 and TGF-beta induced by vitamin D deficiency. Notably, we found the expression of vitamin D receptor significantly increased after LF supplement in colon of vitamin D deficient mice. At the same time, LF reduced the expression of TLR-4, inhibited the activity of NF-kappa B P65 and enhanced the expression of intestinal barrier proteins, including ZO-1, occludin, claudin-1 as well as E-cadherin in the colon of vitamin D deficient mice.