Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion
Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion
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DOI：
10.1016/j.jbiotec.2015.04.004
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发表时间：
2015-12-10
影响因子：
4.1
通讯作者：
Flaschel, Erwin
中科院分区：
文献类型：
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作者：
Grimm, Philipp;Risse, Joe M.;Flaschel, Erwin
在本研究中，研究了利用纤细裸藻的生物量在增值链中生产生育酚、副木兰素和沼气。因此，我们分析了异养、光异养和光自养培养过程中不同生长阶段的细胞干重和产物浓度。此外，还考察了不同来源生物质在产品回收和未回收情况下的比沼气产量。研究表明，生长阶段和培养方式不仅对产物的形成有显著影响，而且还会影响纤细裸藻厌氧消化产气量。异养细胞的最大干重浓度为12.3+/-0.14g L-1，光自养细胞的最大干重浓度为3.4g/-0.02g L-1。异养生物量积累的产物浓度分别为：α-生育酚5.3+/-0.12 mg L-1和副尼龙9.3+/-0.1g L-1或沼气g(-1)80 5+/-10.9m L(每克挥发性固体)。光自养培养细胞的结果为：α-生育酚8.6m g/-0.22m g L-1和副尼龙0.78+/-0.01g L-1或沼气g(-1)648+/-7.2m L。对于节能的下游工序，萃取剂甲醇不一定要严格去除。含有残留甲醇的样品显示沼气产量显著增加，因为这种溶剂可以用作古细菌产生甲烷的额外底物。(C)2015爱思唯尔B.V.保留所有权利。
In this study the use of Euglena gracilis biomass for ce.-tocopherol, paramylon and biogas production in a value-added chain was investigated. Therefore, we analyzed the dry cell weight and product concentrations at different growth phases during heterotrophic, photoheterotrophic and photoautotrophic cultivation in a low-cost minimal medium. Furthermore, the specific biogas yields for differently derived biomass with and without product recovery were investigated. We demonstrate that growth phase and cultivation mode not only have a significant impact on product formation, but also influence the yield of biogas obtained from anaerobic digestion of Euglena gracilis biomass. The maximum dry cell weight concentration ranged from 12.3 +/- 0.14 g L-1 for heterotrophically to 3.4 +/- 0.02 g L-1 for photoautotrophically grown Euglena gracilis cells. The heterotrophically grown biomass accumulated product concentrations of 5.3 +/- 0.12 mg L-1 of alpha-tocopherol and 9.3 +/- 0.1 g L-1 of paramylon or 805 +/- 10.9 mL of biogas g(-1) (per gram volatile solids). The results for photoautotrophically grown cells were 8.6 +/- 0.22 mg L-1 of alpha-tocopherol and 0.78 +/- 0.01 g L-1 of paramylon or 648 +/- 7.2 mL of biogas g(-1). For an energy-saving downstream procedure the extracting agent methanol does not have to be removed strictly. Samples with residual methanol showed a significantly increased biogas yield, because the solvent can be used as an additional substrate for methane production by archaebacteria. (C) 2015 Elsevier B.V. All rights reserved.