SELECTION BY 2 POWERFUL ANTIGENS MAY ACCOUNT FOR THE PRESENCE OF THE MAJOR POPULATION OF HUMAN PERIPHERAL GAMMA DELTA T-CELLS
SELECTION BY 2 POWERFUL ANTIGENS MAY ACCOUNT FOR THE PRESENCE OF THE MAJOR POPULATION OF HUMAN PERIPHERAL GAMMA DELTA T-CELLS
复制标题
DOI：
10.1084/jem.173.6.1311
复制
发表时间：
1991-06-01
影响因子：
15.3
通讯作者：
LANZAVECCHIA, A
中科院分区：
文献类型：
--
作者：
DELIBERO, G;CASORATI, G;LANZAVECCHIA, A
V-γ-9/V-δ-2细胞代表人-γ/δ-细胞的一部分，其在出生后在外周中扩增，携带活化细胞的标志物，并成为外周血中的主要群体。我们发现，这些细胞不包括一个单一的人口，但实际上代表了两个嵌套集，其中较小的，具体的结核分枝杆菌脉冲抗原呈递细胞（APC），包含在一个较大的集特异性抗原上发现的Molt-4淋巴瘤。较大的一组，代表所有血液-γ/δ-细胞的40-80%，由携带V-γ-9/C-γ-1链的细胞组成。在较小的包含组中的细胞对V-delta-2有额外的要求（并且可能对某些允许的连接区域有额外的要求，因为非常小百分比的V-gamma-9/V-delta-2细胞不对分枝杆菌脉冲的APC反应）。分枝杆菌的最佳刺激依赖于APC的存在，并且不受经典的主要组织相容性复合体分子的限制。一些V-gamma-9/V-delta-2分枝杆菌特异性克隆也被不同细菌（如单核细胞增生李斯特菌和大肠杆菌）脉冲的APC刺激，表明该群体包括几种不同的反应模式。这些数据建立了人类特异性和V-γ/V-δ基因使用之间的关系，并为这些γ/δ细胞的外周扩增提供了解释。
V-gamma-9/V-delta-2 cells represent a fraction of human-gamma/delta-cells that is expanded after birth in the periphery, carries markers of activated cells, and becomes a major population in peripheral blood. We found that these cells do not comprise a single population but actually represent two nested sets, the smaller of which, specific for Mycobacterium tuberculosis-pulsed antigen-presenting cells (APC), is contained in a larger set specific for an antigen found on the Molt-4 lymphoma. The larger set, representing 40-80% of all blood-gamma/delta-cells, is comprised of cells bearing the V-gamma-9/C-gamma-1 chain. Cells in the smaller, included set have an additional requirement for V-delta-2(and probably for certain permissive junctional regions, since a very small percentage of V-gamma-9/V-delta-2 cells do not react against mycobacteria-pulsed APC). Optimal stimulation by mycobacteria is dependent on the presence of APC, and is not restricted by classical major histocompatibility complex molecules. Some of the V-gamma-9/V-delta-2 mycobacteria-specific clones are also stimulated by APC pulsed with different bacteria, such as Listeria monocytogenes and Escherichia coli, indicatingthat the population includes several different patterns of reactivity. These data establish a relationship in humans between specificity and V-gamma/V-delta-gene usage, and offer an explanation for the peripheral expansion of these gamma/delta-cells.