Alteration in NMDA receptor subunit mRNA expression in vulnerable and resistant regions of in vitro ischemic rat hippocampal slices
Alteration in NMDA receptor subunit mRNA expression in vulnerable and resistant regions of in vitro ischemic rat hippocampal slices
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DOI：
10.1016/s0304-3940(97)00592-2
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发表时间：
1997-08-29
影响因子：
2.5
通讯作者：
Morley, P
中科院分区：
文献类型：
--
作者：
Small, DL;Poulter, MO;Morley, P
脑损伤，包括脑缺血，可以改变易感神经元中谷氨酸受体亚基的表达。了解谷氨酸受体的这些缺血后变化可以增强我们识别特定的、新的神经保护化合物的能力。采用逆转录聚合酶链反应（RT-PCR）技术，定量测定体外氧糖剥夺后大鼠海马耐药区和易损区n-甲基- d -天冬氨酸（NMDA） NR2A、NR2B和NR2C亚基相对表达的变化。再氧合90分钟后，回到10 mM葡萄糖后，整个切片中NR2C相对于NR2B和NR2A的表达明显增加，选择性易损CA1区和抗性CA3区和齿状回区NR2C的表达也明显增加。(C) 1997爱思唯尔科学爱尔兰有限公司
Brain insults, including cerebral ischemia, can alter glutamate receptor subunit expression in vulnerable neurons. Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used to quantify the altered expression of the N-methyl-D-aspartate (NMDA) NR2A, NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen-glucose deprivation. Ninety minutes after re-oxygenation and return to 10 mM glucose, there was a significant increase in the expression of NR2C relative to NR2B and NR2A in the slice as a whole, as well as in the selectively vulnerable CA1 region and the resistant CA3 and dentate gyrus regions. (C) 1997 Elsevier Science Ireland Ltd.