Vesicular glutamate transporter DNPI/VGLUT2 mRNA is present in C1 and several other groups of brainstem catecholaminergic neurons
Vesicular glutamate transporter DNPI/VGLUT2 mRNA is present in C1 and several other groups of brainstem catecholaminergic neurons
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DOI：
10.1002/cne.10141
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发表时间：
2002-03-12
影响因子：
2.5
通讯作者：
Guyenet, PG
中科院分区：
文献类型：
--
作者：
Stornetta, RL;Sevigny, CP;Guyenet, PG
最近对小鼠谷氨酸囊泡转运蛋白 VGLUT2 进行了表征。在本研究中，使用地高辛标记的 DNPI/VGLUT2 cRNA 探针检查了 VGLUT2 的大鼠同源物，即分化相关的 Na+/P-i 协同转运蛋白 (DNPI)，以确定不同组的桥脑或髓质单胺能神经元中哪些（如果有）表达 DNPI/VGLUT2 mRNA，因此可能是潜在的。 谷氨酸能。 DNPI/VGLUT2 mRNA 广泛分布于脑干内，并且似乎仅是神经元的。通过使用双原位杂交方法，DNPI/VGLUT2和谷氨酸脱羧酶(GAD)-67的mRNA的存在是相互排斥的。通过结合 DNPI/VGLUT2 mRNA 检测和常规免疫组化，在下脑干胆碱能和血清素能细胞中检测不到 DNPI/VGLUT2 mRNA，但在几个酪氨酸羟化酶免疫反应性 (TH-ir) 细胞组中存在。在 C1、C2 和 C3 组的大多数肾上腺素能神经元（TH-ir 神经元的 75-80%）、A2 去甲肾上腺素能组（80%）以及大量的后区细胞中检测到 DNPI/VGLUT2 mRNA。在 A1 区域内，含有 DNPI/VGLUT2 的 TH-ir 细胞要少得多 (16%)。最后，在脑桥去甲肾上腺素能细胞组（A5 和 A6/蓝斑）中检测不到 DNPI/VGLUT2 mRNA。总之，DNPI/VGLUT2 表达的一般模式及其从 GABA 能、胆碱能和血清素能神经元中排除的情况支持 DNPI/VGLUT2 mRNA 识别下脑干中谷氨酸能神经元子集的观点。在该区域内，一些儿茶酚胺能细胞群似乎具有谷氨酸能，包括但不限于肾上腺素能细胞群C1-C3。根据目前的证据，脑桥的去甲肾上腺素能细胞群（A5 和 A6）不包含任何一种已知的囊泡谷氨酸转运蛋白，而且很可能也不包含。谷氨酸能。 J.Comp。内罗尔。 444:191-206, 2002。(C) 2002 Wiley-Liss, Inc.
The mouse glutamate vesicular transporter VGLUT2 has recently been characterized. The rat homolog of VGLUT2, differentiation-associated Na+/P-i cotransporter (DNPI), was examined using a digoxigenin-labeled DNPI/VGLUT2 cRNA probe in the present study to determine which, if any, of the various groups of pontine or medullary monoaminergic neurons express DNPI/VGLUT2 mRNA and, thus, are potentially glutamatergic. DNPI/VGLUT2 mRNA was widely distributed within the brainstem and seemed exclusively neuronal. By using a double in situ hybridization method, the presence of the mRNA for DNPI/VGLUT2 and glutamic acid decarboxylase (GAD)-67 was mutually exclusive. By combining DNPI/VGLUT2 mRNA detection and conventional immunohistochemistry, DNPI/VGLUT2 mRNA was undetectable in lower brainstem cholinergic and serotonergic cells, but it was present in several tyrosine hydroxylase-immunoreactive (TH-ir) cell groups. DNPI/VGLUT2 mRNA was detected in most of the adrenergic neurons of the C1, C2, and C3 groups (75-80% of TH-ir neurons), in the A2 noradrenergic group (80%), and in vast numbers of area postrema cells. Within the A1 region, many fewer TH-ir cells contained DNPI/VGLUT2 (16%). Finally, DNPI/VGLUT2 mRNA was undetectable in the pontine noradrenergic cell groups (A5 and A6/locus coeruleus). In conclusion, the general pattern of DNPI/VGLUT2 expression and its exclusion from GABAergic, cholinergic, and serotonergic neurons supports the notion that DNPI/VGLUT2 mRNA identifies a subset of glutamatergic neurons in the lower brainstem. Within this region several catecholaminergic cell groups appear to be glutamatergic, including but not limited to the adrenergic cell groups C1-C3. Based on the present evidence, the noradrenergic cell groups of the pons (A5 and A6) do not contain either known vesicular glutamate transporter and are most likely not. glutamatergic. J. Comp. Neurol. 444:191-206, 2002. (C) 2002 Wiley-Liss, Inc.