Automatic alignment and reconstruction of images for soft X-ray tomography.
Automatic alignment and reconstruction of images for soft X-ray tomography.
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DOI：
10.1016/j.jsb.2011.11.027
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发表时间：
2012-02
影响因子：
3
通讯作者：
Le Gros, Mark A.
中科院分区：
文献类型：
--
作者：
Parkinson, Dilworth Y.;Knoechel, Christian;Yang, Chao;Larabell, Carolyn A.;Le Gros, Mark A.
软X射线断层扫描（SXT）是一种强大的成像技术，可生成近天然状态下全细胞结构组织的定量3D图像。SXT也是一种高通量成像技术。在国家X射线断层扫描中心（NCXT），整个细胞的断层扫描重建的标本制备和图像收集只需要几分钟。然而，对齐和重建数据需要更长的时间。在这里，我们描述了一个新的组件的高吞吐量的计算管道用于处理数据在NCXT。我们已经开发出一种新的方法，用于自动对齐投影图像，不需要基准标记或手动与软件交互。这种方法已被优化的SXT数据集，这通常涉及充分旋转的标本。该软件为NCXT SXT仪器的用户提供了一种新的功能-在成像实验期间几乎实时地获得初始3D结果，随后可以进一步改进。新的代码自动重建3D（AREC 3D）也是快速，可靠和强大的。代码的基本架构也适用于高性能GPU处理，这可以显着提高速度和保真度。
Soft x-ray tomography (SXT) is a powerful imaging technique that generates quantitative, 3D images of the structural organization of whole cells in a near-native state. SXT is also a high-throughput imaging technique. At the National Center for X-ray Tomography (NCXT), specimen preparation and image collection for tomographic reconstruction of a whole cell require only minutes. Aligning and reconstructing the data, however, take significantly longer. Here we describe a new component of the high throughput computational pipeline used for processing data at the NCXT. We have developed a new method for automatic alignment of projection images that does not require fiducial markers or manual interaction with the software. This method has been optimized for SXT data sets, which routinely involve full rotation of the specimen. This software gives users of the NCXT SXT instrument a new capability - virtually real-time initial 3D results during an imaging experiment, which can later be further refined. The new code, Automatic Reconstruction 3D (AREC3D), is also fast, reliable and robust. The fundamental architecture of the code is also adaptable to high performance GPU processing, which enables significant improvements in speed and fidelity.
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影响因子：
2.2
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通讯作者：
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