INDUCTION OF CYTOKINE PRODUCTION FROM HUMAN MONOCYTES STIMULATED WITH ALGINATE
INDUCTION OF CYTOKINE PRODUCTION FROM HUMAN MONOCYTES STIMULATED WITH ALGINATE
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DOI：
10.1097/00002371-199108000-00007
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发表时间：
1991-08-01
影响因子：
3.9
通讯作者：
ESPEVIK, T
中科院分区：
文献类型：
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作者：
OTTERLEI, M;OSTGAARD, K;ESPEVIK, T
藻酸盐是具有凝胶形成特性的多糖，由1，4-连接的β-D-甘露糖醛酸（M）、α-L-古罗糖醛酸（G）和交替（MG）嵌段组成。藻酸盐可用作体内植入细胞的基质。在这项研究中，我们研究了藻酸盐及其组分刺激人单核细胞产生肿瘤坏死因子-α、白细胞介素-6和白细胞介素-1的能力。藻酸盐刺激单核细胞产生高水平的所有三种细胞因子。与高G藻酸盐相比，低G藻酸盐诱导细胞因子产生的效力高约10倍。M-阻断剂和MG-阻断剂刺激细胞因子产生，但G-阻断剂不刺激。结果表明，甘露糖醛酸残基是藻酸盐中的活性细胞因子诱导剂。
Alginates are polysaccharides with gel-forming properties composed of 1,4-linked beta-D-mannuronic acid (M), alpha-L-guluronic acid (G), and alternating (MG) blocks. Alginate can be used as a matrix for implanted cells in vivo. In this study, we have examined the ability of alginates and their components to stimulate human monocytes to produce tumor necrosis factor-alpha, interleukin-6, and interleukin-1. Alginates stimulated the monocytes to produce high levels of all three cytokines. Low G alginates were approximately 10 times more potent in inducing cytokine production compared with high G alginates. The M-blocks and the MG-blocks, but not the G-blocks, stimulated the cytokine production. The results demonstrate that the mannuronic acid residues are the active cytokine inducers in alginates.