EDITING OF A TRANSFER-RNA ANTICODON IN MARSUPIAL MITOCHONDRIA CHANGES ITS CODON RECOGNITION
EDITING OF A TRANSFER-RNA ANTICODON IN MARSUPIAL MITOCHONDRIA CHANGES ITS CODON RECOGNITION
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DOI：
10.1093/nar/21.7.1523
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发表时间：
1993-04-11
影响因子：
14.9
通讯作者：
PAABO, S
中科院分区：
文献类型：
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作者：
JANKE, A;PAABO, S
RNA编辑已被描述为原生动物和植物的细胞器基因，以及哺乳动物的核转录物的三种情况。天冬氨酸的线粒体tRNA在有袋动物的线粒体基因组中编码，反密码子为GCC。然而，来自基因产物的cDNA揭示了tRNA的反密码子的第二个核苷酸在转录后改变为被识别为尿苷残基。这恢复了tRNA的编码特性。直接测序以及扩增的cDNA的克隆表明，约50%的天冬氨酸前体tRNA分子的细胞库被编辑。因此，RNA编辑发生在哺乳动物线粒体中，并且可以影响tRNA的反密码子。
RNA editing has been described for organellar genes of protozoa and plants as well as in three cases for nuclear transcipts of mammals. The mitochondrial tRNA for aspartic acid is encoded in the mitochondrial genome of marsupials with the anticodon GCC. However, cDNA from the gene product revealed that the second nucleotide of the anticodon of the tRNA is posttranscriptionally changed to be recognized as an uridine residue. This restores the coding property of the tRNA. Direct sequencing as well as clones of the amplified cDNA indicated that about 50% of the cellular pool of pre-tRNA molecules for aspartic acid is edited. Thus, RNA editing occurs in mammalian mitochondria and can affect the anticodons of tRNAs.