Insights Obtained by Culturing Saccharibacteria With Their Bacterial Hosts
Insights Obtained by Culturing Saccharibacteria With Their Bacterial Hosts
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DOI：
10.1177/0022034520905792
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发表时间：
2020-02-19
影响因子：
7.6
通讯作者：
Dewhirst, F. E.
中科院分区：
文献类型：
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作者：
Bor, B.;Collins, A. J.;Dewhirst, F. E.
口腔微生物组研究已经从问“谁在那里？”“到“他们在做什么？了解微生物“做什么”涉及多种方法，包括获得基因组信息和检查物种间的相互作用。最近，我们分离出一种人类口腔链球菌（TM 7）细菌，HMT-952，菌株TM 7 x，这是一种超小的口腔细菌放线菌的寄生虫。宿主-寄生虫相互作用，如噬菌体-细菌或Saccharomyces细菌-宿主细菌，是研究不足的领域，具有很大的洞察力。Saccharobacteria门是候选辐射门（Candidate Phyla Radiation）的成员，这是一个以前没有培养成员的大谱系。然而，扩大我们对Saccharomyces细菌-宿主相互作用的理解需要检查多个遗传学上不同的Saccharomyces细菌-宿主对。在这里，我们报告了3个额外的Saccharomyces细菌物种的分离，从人类口腔中的二元共培养与他们的细菌宿主。它们是通过过滤不含其他较大细菌的超小Saccharomyces细菌细胞并将其接种到潜在宿主细菌的培养物中而获得的。获得的二元共培养物可以稳定传代和研究。获得完整的封闭基因组，并允许进行全基因组分析。都有小基因组（
Oral microbiome research has moved from asking "Who's there?" to "What are they doing?" Understanding what microbes "do" involves multiple approaches, including obtaining genomic information and examining the interspecies interactions. Recently we isolated a human oral Saccharibacteria (TM7) bacterium, HMT-952, strain TM7x, which is an ultrasmall parasite of the oral bacterium Actinomyces odontolyticus. The host-parasite interactions, such as phage-bacterium or Saccharibacteria-host bacterium, are understudied areas with large potential for insight. The Saccharibacteria phylum is a member of Candidate Phyla Radiation, a large lineage previously devoid of cultivated members. However, expanding our understanding of Saccharibacteria-host interactions requires examining multiple phylogenetically distinct Saccharibacteria-host pairs. Here we report the isolation of 3 additional Saccharibacteria species from the human oral cavity in binary coculture with their bacterial hosts. They were obtained by filtering ultrasmall Saccharibacteria cells free of other larger bacteria and inoculating them into cultures of potential host bacteria. The binary cocultures obtained could be stably passaged and studied. Complete closed genomes were obtained and allowed full genome analyses. All have small genomes (