Magnetic resonance imaging of cardiomyocyte apoptosis with a novel magneto-optical nanoparticle
Magnetic resonance imaging of cardiomyocyte apoptosis with a novel magneto-optical nanoparticle
复制标题
DOI：
10.1002/mrm.20617
复制
发表时间：
2005-09-01
影响因子：
3.3
通讯作者：
Josephson, L
中科院分区：
文献类型：
--
作者：
Sosnovik, DE;Schellenberger, EA;Josephson, L
利用高分辨率MRI对体内心肌细胞凋亡进行成像的能力可以促进新的心脏保护疗法的发展。因此，在体外将新型纳米颗粒AnxCLIO-Cy 5.5对心肌细胞凋亡的敏感性与膜联蛋白V-FITC的敏感性进行比较，并显示出高度的共定位。然后在短暂冠状动脉（LAD）闭塞后，在给予AnxCLIO-Cy 5.5的五只小鼠和给予相同剂量（2 mg Fe/kg）的CLIO-Cy 5.5的四只小鼠中进行MRI。MR信号强度和心肌T-2* 进行了评价，在体内，在心肌运动功能减退的区域在LAD分布。进行离体荧光成像以确认体内发现。给予AnxCLIO-Cy 5.5的小鼠的心肌T2* 显著降低（8.1 vs. 13.2ms，P < 0.01），并且荧光靶与背景比率显著升高（2.1 vs. 1.1，P < 0.01）。因此，本研究证明了用新型纳米颗粒AnxCLIO-Cy 5.5在体内获得心肌细胞凋亡的高分辨率MR图像的可行性。
The ability to image cardiomyocyte apoptosis in vivo with high-resolution MRI could facilitate the development of novel cardioprotective therapies. The sensitivity of the novel nanoparticle AnxCLIO-Cy5.5 for cardiomyocyte apoptosis was thus compared in vitro to that of annexin V-FITC and showed a high degree of colocalization. MRI was then performed, following transient coronary artery (LAD) occlusion, in five mice given AnxCLIO-Cy5.5 and in four mice given an identical dose (2 mg Fe/kg) of CLIO-Cy5.5. MR signal intensity and myocardial T-2* were evaluated, in vivo, in hypokinetic regions of myocardium in the LAD distribution. Ex vivo fluorescence imaging was performed to confirm the in vivo findings. Myocardial T2* was significantly lower in the mice given AnxCLIO-Cy5.5 (8.1 versus 13.2 ms, P < 0.01), and fluorescence target to background ratio was significantly higher (2.1 versus 1.1, P < 0.01). This study thus demonstrates the feasibility of obtaining high-resolution MR images of cardiomyocyte apoptosis in Vivo with the novel nanoparticle, AnxCLIO-Cy5.5.