Role of the histidine kinase, EnvZ, in the production of outer membrane proteins in the symbiotic-pathogenic bacterium Xenorhabdus nematophilus
Role of the histidine kinase, EnvZ, in the production of outer membrane proteins in the symbiotic-pathogenic bacterium Xenorhabdus nematophilus
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DOI：
10.1128/aem.63.3.962-968.1997
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发表时间：
1997-03-01
影响因子：
4.4
通讯作者：
Tabatabai, N
中科院分区：
文献类型：
--
作者：
Forst, SA;Tabatabai, N
我们发现，envZ（编码嗜热短杆菌组氨酸激酶传感器蛋白EnvZ的基因）的失活影响了几种外膜蛋白（Opns）的产生，X。EnvZ的插入失活导致OppN的产生减少，OppN是构成X中总外膜蛋白的约50%的OmpF样孔形成蛋白。奥菲勒斯在向稳定期过渡期间，野生型菌株中诱导了三种新的外膜蛋白OpnB、OpnS和OpnX。EnvZ-阴性菌株ANT1不产生OpnB和OpnX，而OpnS以显著降低的水平被诱导。这些结果表明EnvZ是指数生长期间OpnB的高水平产生所必需的，并且可能参与稳定期生长期间OpnB、OpnS和OpnX的产生。我们还表明，ANT1比野生型菌株的致病性更强时，只有5个细胞被注射到血淋巴的幼虫阶段的烟草天蛾（Manduca serta）。在血淋巴中检测到大量细菌之前，幼虫死亡。这些结果与EnvZ在嗜热假单胞菌生活史中的作用有关。
We show that inactivation of envZ, the gene encoding the histidine kinase sensor protein, EnvZ, of Xenorhabdus nematophilus, affected the production of several outer membrane proteins (Opns), X. nematophilus produced five major Opns during exponential growth, Insertional inactivation of envZ led to a decrease in the production of OpnP, the OmpF-like pore-forming protein which constitutes approximately 50% of the total outer membrane protein in X. nematophilus. OpnA production was also reduced, while the remaining Opns were produced normally, During the transition to stationary phase, three new outer membrane proteins, OpnB, OpnS, and OpnX, were induced in the wild-type strain. The envZ-minus strain, ANT1, did not produce OpnB and OpnX, while OpnS was induced at markedly reduced levels, These results suggest that EnvZ was required for the high-level production of OpnP during exponential growth and may be involved in the production of OpnB, OpnS, and OpnX during stationary-phase growth, We also show that ANT1 was more pathogenic than the wild-type strain when as few as five cells were injected into the hemolymph of the larval stage of the tobacco hornworm (Manduca serta). The larvae died before significant numbers of bacteria were detectable in the hemolymph, These results are discussed in relation to the role of EnvZ in the life cycle of X, nematophilus.