Effects of Culturing on the Stability of the Putative Murine Adipose Derived Stem Cells Markers.
Effects of Culturing on the Stability of the Putative Murine Adipose Derived Stem Cells Markers.
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DOI：
10.2174/1876893800901010054
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Niyibizi C
中科院分区：
文献类型：
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作者：
Maddox JR;Liao X;Li F;Niyibizi C
间充质干细胞因其在再生医学中的潜在应用而引起了广泛的关注。在这些细胞的应用中，主要的缺点是没有一个或多个单一的标记来识别和帮助将这些细胞从各种其他细胞类型中分离出来。通常表达的间充质干细胞表面抗原包括CD44、CD73、CD90.2、CD105和CD146。在本研究中，我们研究了这些表面抗原在培养中的稳定性及其在鉴定和分离鼠源性脂肪源性干细胞中的潜在应用。数据表明，随着培养的增加，这些标记的表达量增加，到第8代时趋于稳定；在这一传代中，细胞的表面标记物进行了阳性分选。每个亚群在培养中保持，并在体外和体内评估向成骨谱系的分化。CD73和CD105阳性细胞亚群在体外表现出向成骨谱系的强大分化；CD90.2+细胞亚群向成骨谱系分化程度最低。对体内分化的细胞亚群的评估表明，所有的细胞亚群都表现出分化成成骨细胞的潜力。综上所述，这些数据表明，尽管这组标记可用于鉴定具有向成骨谱系分化潜力的细胞，但不能用于从各种其他细胞类型中富集ADSC。
Mesenchymal stem cells have generated much interest because of their potential use in regenerative medicine. The major draw back in the application of these cells is that there is no single marker or markers that have been established to identify and aid in isolating the cells from a variety of other cell types. The commonly expressed mesenchymal stem cell surface antigens include CD44, CD73, CD90.2, CD105, and CD146. In the present study we examined the stability of these surface antigens in culture and their potential application in identifying and isolating murine derived adipose derived stem cells. The data showed that the expression of these markers increased with culturing and appeared to stabilize by passage 8; the cells were sorted positively for the surface markers at this passage. Each subset was maintained in culture and evaluated for differentiation toward osteogenic lineage in vitro and in vivo. The CD73 and CD105 positive cell subsets demonstrated robust differentiation toward osteogenic lineage in vitro; the CD90.2+ cell subset exhibited the least differentiation toward osteogenic lineage. Assessment of the cell subpopulations for in vivo differentiation demonstrated that all the cell subsets exhibited potential to differentiate into osteoblasts. Taken together, these data suggest that this panel of markers although useful in identifying cells with potential to differentiate toward osteogenic lineage, cannot prospectively be used for enriching for ADSC from a variety of other cell types.