Hematologic, cytochemical, ultrastructural, and molecular findings of Hepatozoon-infected flat-headed cats (Prionailurus planiceps).
Hematologic, cytochemical, ultrastructural, and molecular findings of Hepatozoon-infected flat-headed cats (Prionailurus planiceps).
复制标题
肝虫感染的平头猫（Prionailurus planiceps）的血液学、细胞化学、超微结构和分子发现。
DOI：
10.1111/j.1939-165x.2008.00011.x
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发表时间：
2008
影响因子：
1.2
通讯作者：
R. Pattanarangsan
中科院分区：
文献类型：
--
作者：
C. Salakij;J. Salakij;N. Narkkong;T. Sirinarumitr;R. Pattanarangsan
背景
平头猫（Prionailurus planiceps）是东南亚的一种小型野猫，被认为是极度濒危物种。关于该物种相对于其他猫科动物的血液学价值、血细胞形态或血液寄生虫知之甚少。
目标
本研究的目的是报告 2 只野生捕获的平头猫的基本血液学值并描述血细胞的光学显微镜、细胞化学和超微结构特征。此外，还对两只猫的中性粒细胞中发现的肝虫生物体进行了分子分析。
方法
从头静脉将血样收集到 EDTA 中。使用标准方法进行 CBC、手动分类计数、手动网织红细胞计数、细胞化学染色（苏丹黑 B [SBB]、α-萘乙酸酯酶 [ANAE] 和 β-葡萄糖醛酸酶）以及扫描和透射电子显微镜检查。
结果
HCT 略低，网织红细胞计数和红细胞分布宽度高于其他品种猫的预期值。两只猫的中性粒细胞胞质中均发现肝虫生物，但受感染的中性粒细胞数量非常低（1%-2%）。中性粒细胞对 SBB 染色呈强阳性，但对 ANAE 和 β-葡萄糖醛酸酶染色呈阴性。肝虫感染的中性粒细胞 SBB 呈阴性，但 ANAE 和 β-葡萄糖醛酸酶局部呈阳性。透射电镜观察发现，中性粒细胞中可见肝原虫配子，血浆中很少游离。与未感染的中性粒细胞相比，感染的中性粒细胞具有更少的特异性颗粒和更多的线粒体。部分18S rRNA PCR产物显示，平头猫体内分离的肝虫与青蛙肝虫密切相关。
结论
这些结果增加了我们对感染肝虫感染的平头猫的血液学值和血细胞形态以及肝虫生物体的分子分析的了解，并可能有助于该物种血液寄生虫病的健康管理和评估。
BACKGROUND
The flat-headed cat (Prionailurus planiceps) is a small wild cat of Southeast Asia and is considered extremely endangered. Little is known about the hematologic values, blood cell morphology, or hemoparasites of this species in relation to other Felidae.
OBJECTIVES
The objective of this study was to report basic hematologic values and describe the light microscopic, cytochemical, and ultrastructural characteristics of blood cells in 2 wild-caught flat-headed cats. In addition, molecular analysis was done of a Hepatozoon organism found in the neutrophils of both cats.
METHODS
Blood samples were collected into EDTA from the cephalic vein. A CBC, manual differential count, manual reticulocyte count, cytochemical stains (Sudan black B [SBB], alpha-naphthyl acetate esterase [ANAE], and beta-glucuronidase), and scanning and transmission electron microscopy were done using standard methods.
RESULTS
HCT was slightly lower and reticulocyte counts and red cell distribution width were higher than the expected values for other species of cats. Hepatozoon organisms were found in the cytoplasm of neutrophils in both cats, but the number of infected neutrophils was very low (1%-2%). Neutrophils stained strongly positive for SBB, but were negative for ANAE and beta-glucuronidase. Hepatozoon-infected neutrophils were negative for SBB, but focally positive for ANAE and beta-glucuronidase. By transmission electron microscopy, gamonts of Hepatozoon sp were observed in neutrophils, and rarely free in plasma. Infected neutrophils had fewer specific granules and more mitochondria compared with noninfected neutrophils. PCR products of partial 18S rRNA revealed that the isolate of Hepatozoon in the flat-headed cats was closely related to that of the frog Hepatozoon sp.
CONCLUSIONS
These results add to our understanding of hematologic values and blood cell morphology in Hepatozoon-infected flat-headed cats as well as the molecular analysis of the Hepatozoon organism, and may be useful for the health management and evaluation of hemoparasitic disease in this species.