Characterization of influenza virus sialic acid receptors in minor poultry species
Characterization of influenza virus sialic acid receptors in minor poultry species
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DOI：
10.1186/1743-422x-7-365
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发表时间：
2010-12-09
期刊：
影响因子：
4.8
通讯作者：
Perez, Daniel R.
中科院分区：
文献类型：
--
作者：
Kimble, Brian;Ramirez Nieto, Gloria;Perez, Daniel R.
通常认为，禽流感病毒（AIV）结合末端α 2，3唾液酸（SA）残基，而人流感病毒结合α 2，6 SA残基。通过HA表面蛋白上的一系列氨基酸改变，AIV可以转换受体特异性并识别α 2，6 SA阳性细胞，包括人呼吸道上皮细胞。动物物种，如猪和日本鹌鹑，含有α 2，3和α 2，6 SA成为受体转换的理想环境。本文描述了北京鸭、图卢兹鹅、环颈雉、白色小火鸡、白色鹌鹑和珍珠珍珠鸡等6种常见小型家禽的唾液酸模式和分布。用α 2，3和α 2，6 SA特异性凝集素（分别为Maakia amurensis凝集素和Sambuca nigra凝集素）通过基于碱性磷酸酶的方法和基于荧光的方法检测SA。通过荧光标记已知对一种受体或另一种具有特异性的4种不同H3N2流感病毒，可见SA部分的差异及其结合流感病毒的能力。鹅和鸭在整个呼吸道中显示α 2，3 SA，仅在结肠中显示边缘α 2，6 SA。其他四种鸟类在呼吸道和肠道中都显示出α 2，3和α 2，6 SA。此外，火鸡呼吸道显示年龄与α 2，6 SA水平呈正相关。这些鸟类同时具有禽流感和人类流感受体的事实，加上它们在后院农场和世界各地的活禽市场中的普遍存在，将它们标记为潜在的混合碗物种，并且需要改进对这些鸟类在流感宿主转换中的作用的监测和额外的研究。
It is commonly accepted that avian influenza viruses (AIVs) bind to terminal alpha 2,3 sialic acid (SA) residues whereas human influenza viruses bind to alpha 2,6 SA residues. By a series of amino acid changes on the HA surface protein, AIVs can switch receptor specificity and recognize alpha 2,6 SA positive cells, including human respiratory epithelial cells. Animal species, like pigs and Japanese quail, that contain both alpha 2,3 and alpha 2,6 SA become ideal environments for receptor switching. Here, we describe the SA patterns and distributions in 6 common minor domestic poultry species: Peking duck, Toulouse geese, Chinese ring-neck pheasant, white midget turkey, bobwhite quail, and pearl guinea fowl. Lectins specific to alpha 2,3 and alpha 2,6 SA (Maakia amurensis agglutinin and Sambuca nigra agglutinin, respectively) were used to detect SA by an alkaline phosphotase-based method and a fluorescent-based method. Differences in SA moieties and their ability to bind influenza viruses were visualized by fluorescent labeling of 4 different H3N2 influenza viruses known to be specific for one receptor or the other. The geese and ducks showed alpha 2,3 SA throughout the respiratory tract and marginal alpha 2,6 SA only in the colon. The four other avian species showed both alpha 2,3 and alpha 2,6 SA in the respiratory tract and the intestines. Furthermore, the turkey respiratory tract showed a positive correlation between age and alpha 2,6 SA levels. The fact that these birds have both avian and human flu receptors, combined with their common presence in backyard farms and live bird markets worldwide, mark them as potential mixing bowl species and necessitates improved surveillance and additional research about the role of these birds in influenza host switching.