Dopaminergic neuronal loss in transgenic mice expressing the Parkinson's disease-associated UCH-L1 193M mutant
Dopaminergic neuronal loss in transgenic mice expressing the Parkinson's disease-associated UCH-L1 193M mutant
复制标题
DOI：
10.1016/j.neuint.2006.07.015
复制
发表时间：
2007-01-01
影响因子：
4.2
通讯作者：
Wada, Keiji
中科院分区：
文献类型：
--
作者：
Setsuie, Rieko;Wang, Yu-Lai;Wada, Keiji
The 193M mutation in ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) was reported in one German family with autosomal dominant Parkinson's disease (PD). The causative role of the mutation has, however, been questioned. We generated transgenic (Tg) mice carrying human UCHL1 under control of the PDGF-B promoter; two independent lines were generated with the 193M mutation (a high- and low-expressing line) and one line with wild-type human UCH-L1. We found a significant reduction in the dopaminergic neurons in the substantia nigra and the dopamine content in the striatum in the high-expressing 193M Tg mice as compared with non-Tg mice at 20 weeks of age. Although these changes were absent in the low-expressing 193M Tg mice, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment profoundly reduced dopaminergic neurons in this line as compared with wild-type Tg or non-Tg mice. Abnormal neuropathologies were also observed, such as silver staining-positive argyrophilic grains in the perikarya of degenerating dopaminergic neurons, in 193M Tg mice. The midbrains of 193M Tg mice contained increased amounts of insoluble UCH-L1 as compared with those of non-Tg mice, perhaps resulting in a toxic gain of function. Collectively, our data represent in vivo evidence that expression of UCHL1(193M) leads to the degeneration of dopaminergic neurons. (c) 2006 Elsevier Ltd. All rights reserved.
