The Complete Mitochondrial Genome of Glyptothorax macromaculatus Provides a Well-Resolved Molecular Phylogeny of the Chinese Sisorid Catfishes.
The Complete Mitochondrial Genome of Glyptothorax macromaculatus Provides a Well-Resolved Molecular Phylogeny of the Chinese Sisorid Catfishes.
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Glyptothorax大莫拉图斯的完整线粒体基因组为中国杂菌cat鱼提供了完善的分子系统发育。
作者：
Lv Y;Li Y;Ruan Z;Bian C;You X;Yang J;Jiang W;Shi Q
以往的系统发育分析中国鲶科鲶鱼要么解决不好，或没有包括所有的12个属。在这里，我们成功地组装了第一个完整的线粒体基因组的sisorid鱼Glyptothorax macromaculatus。基于这种新的线粒体基因组和以前发表的线粒体基因组中的Sisoridae，我们产生了最大似然和贝叶斯遗传。我们使用化石校准点确定了我们首选的拓扑结构。我们还测试了蛋白质编码基因的线粒体基因组中的glyptosternoid鱼类的自然选择的信号，通过比较核苷酸替换率沿着的分支祖先的glyptosternoid鱼类在我们的拓扑结构的其他分支。线粒体序列结构分析表明，G. macromaculatus与已知的其他脊椎动物相似，但略有不同。我们的Sisorid系统发育得到了很好的解决和支持，与不同的系统发育方法之间的确切一致性。这一强有力的系统发育阐明了中国科属之间的关系，并有力地支持了该科分为三个主要分支。有趣的是，我们的分子测年分析预测的glyptosternoid分歧时间与青藏高原的隆起相吻合，这表明地质可能影响了Sisoridae的物种形成。在编码线粒体蛋白质的基因中，atp8可能进化得最快，而atp6可能受到了适应高海拔的正选择压力。本研究为中国扁尾鱼科鱼类的发生、进化和高海拔适应性研究提供了新的视角。
Previous phylogenetic analyses of the Chinese sisorid catfishes have either been poorly resolved or have not included all the 12 sisorid genera. Here, we successfully assembled the first complete mitochondrial genome of the sisorid fish Glyptothorax macromaculatus. Based on this novel mitochondrial genome and previously published mitochondrial genomes in the Sisoridae, we generated maximum likelihood and Bayesian phylogenies. We dated our preferred topology using fossil calibration points. We also tested the protein-coding genes in the mitochondrial genomes of the glyptosternoid fishes for signals of natural selection by comparing the nucleotide substitution rate along the branch ancestral to the glyptosternoid fishes to other branches in our topology. The mitochondrial sequence structure of G. macromaculatus was similar to those known from other vertebrates, with some slight differences. Our sisorid phylogenies were well-resolved and well-supported, with exact congruence between the different phylogenetic methods. This robust phylogeny clarified the relationships among the Chinese sisorid genera and strongly supported the division of the family into three main clades. Interestingly, the glyptosternoid divergence time predicted by our molecular dating analysis coincided with the uplift of the Tibetan Plateau, suggesting that geology may have influenced speciation in the Sisoridae. Among the mitochondrial protein-coding genes, atp8 may have most rapidly evolved, and atp6 may have been subjected to positive selection pressure to adapt to high elevations. In summary, this study provided novel insights into the phylogeny, evolution and high-altitude adaptions of the Chinese sisorid fishes.
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