Fragmentation of landscape as a cause for genetic subdivision in bank voles
Fragmentation of landscape as a cause for genetic subdivision in bank voles
复制标题
DOI：
10.1046/j.1523-1739.2000.98519.x
复制
发表时间：
2000-08-01
影响因子：
6.3
通讯作者：
Musolf, K
中科院分区：
文献类型：
--
作者：
Gerlach, G;Musolf, K
研究了不同道路对河岸田鼠（Clethrionomys glareolus）种群遗传分化的影响。根据7个微卫星标记的多态性，计算了自然群体的等位基因频率、遗传变异和遗传距离。我们比较了银行田鼠种群在控制区没有这样的障碍，动物从一个国家的道路，铁路和高速公路两侧。使用F和R统计，我们证明了显着的人口细分银行田鼠人口分开的高速公路，但不是在人口的任何一方的其他道路或在控制区。通过基于Mantel分析的扩展方法揭示了地理距离和遗传距离之间的相关性。这使我们能够测量遗传屏障效应，并将其表示为额外的地理距离。例如，被莱茵河和康斯坦茨湖分隔的德国南部和瑞士的人群中，所有七个基因座的等位基因频率存在统计学显著差异。康斯坦茨和瑞士伦维尔的河岸田鼠种群之间的真实的地理距离为6 km。根据这一分析，莱茵河的遗传屏障效应可被定义为7.7公里的额外距离。这项研究首次表明，不仅旧的地理障碍，而且最近的景观破碎，例如，高速公路对基因流和遗传亚结构的人口，这应该考虑在未来的环境影响评估的重要影响。
We studied the barrier effects of various roadways on the genetic subdivision of bank vole (Clethrionomys glareolus) populations. Allele frequencies, genetic variability, and genetic distances of natural populations were calculated based on polymorphism of seven microsatellite markers. We compared bank vole populations in control areas without such barriers with animals from both sides of a country road, a railway, and a highway. Using F and R statistics, we demonstrated significant population subdivision in bank vole populations separated by the highway, but not in populations on either side of the other roadways or in the control area. Correlations between geographic and genetic distances were revealed by an extended method based on a Mantel analysis. This allowed us to measure genetic barrier effects and express them as additional geographic distances. For instance, statistically significant differences in allele frequencies in all seven loci examined existed among populations in southern Germany and Switzerland, which are separated by the Rhine River and Lake Constance. The real geographic distance between bank vole populations in Konstanz and those in Lengwil, Switzerland, was 6 km. According to this analysis the genetic barrier effect of the Rhine could be defined as an additional distance of 7.7 km. This study shows for the first time that not only old geographic barriers but also more recent fragmentation of landscape by, for example, highways has an important effect on gene flow and the genetic substructuring of populations, which should be considered in future environmental impact assessments.