Skeletal muscle wasting and contractile performance in septic rats
Skeletal muscle wasting and contractile performance in septic rats
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DOI：
10.1002/mus.20268
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发表时间：
2005-03-01
期刊：
影响因子：
3.4
通讯作者：
Hesselink, MKC
中科院分区：
文献类型：
--
作者：
Minnaard, R;Drost, MR;Hesselink, MKC
我们研究了脓毒症对肌肉消耗和功能的时间影响，以研究肌肉消耗对肌肉功能下降的影响；我们还研究了这种肌肉萎缩的纤维类型特异性。通过向大鼠腹膜内注射酵母聚糖悬浮液来诱导脓毒症。注射后 2 小时以及第 2、6 和 11 天，使用原位电刺激测量肌肉功能。与配对喂养和随意喂养对照相比，酵母聚糖注射引起严重的肌肉萎缩。第 6 天，酵母聚糖处理的大鼠的等长力产生能力急剧下降。我们的结论是，这完全是由肌肉质量的减少造成的。在第 6 天，我们还观察到脓毒症大鼠腓肠肌中 20S 蛋白酶体的活性增加，但比目鱼肌中的活性没有增加。在胫骨前肌中，但不在比目鱼肌中，肌肉萎缩以纤维类型特定的方式发生，即，1 型纤维横截面积的减少明显小于 2A 型和 2B/X 型纤维。这些发现表明肌肉的固有功能和肌纤维类型分布都会影响对分解代谢信号的反应。
We investigated the temporal effects of sepsis on muscle wasting and function in order to study the contribution of wasting to the decline in muscle function; we also studied the fiber-type specificity of this muscle wasting. Sepsis was induced by injecting rats intraperitoneally with a zymosan suspension. At 2 h and at 2, 6, and 11 days after injection, muscle function was measured using in situ electrical stimulation. Zymosan injection induced severe muscle wasting compared to pair-fed and ad libitum fed controls. At 6 days, isometric force-generating capacity was drastically reduced in zymosan-treated rats. We conclude that this was fully accounted for by the reduction of muscle mass. At day 6, we also observed increased activity of the 20S proteasome in gastrocnemius but not soleus muscle from septic rats. In tibialis anterior but not in soleus, muscle wasting occurred in a fiber-type specific fashion, i.e., the reduction in cross-sectional area was significantly smaller in type 1 than type 2A and 2B/X fibers. These findings suggest that both the inherent function of a muscle and the muscle fiber-type distribution affect the responsiveness to catabolic signals.