Not "out of Nantucket": Babesia microti in southern New England comprises at least two major populations
Not "out of Nantucket": Babesia microti in southern New England comprises at least two major populations
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DOI：
10.1186/s13071-014-0546-y
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发表时间：
2014-12-10
影响因子：
3.2
通讯作者：
Telford, Sam R., III
中科院分区：
文献类型：
--
作者：
Goethert, Heidi K.;Telford, Sam R., III
背景资料：鹿蜱传播的人类巴贝虫病由于Babelicoti似乎正在扩大其分布和流行在美国东北部。一个假设，这种出现是引入寄生虫到新的网站从长期的传播领域，如南图克特岛，马萨诸塞州。方法：我们开发了一个分型系统的基础上，区分个人B的可变数目串联重复基因座。microti基因型。因此，我们分析了来自11个地点的寄生虫的种群结构，代表了新英格兰南部（美国东北部）的长期和新出现的传播，并比较了它们的单倍型和等位基因频率，以确定最可能的B数量。以我们的地方病标本为代表的田鼠种群。我们希望能找到证据证明新英格兰南部的点源传入，所有的寄生虫都明显来自南图克特岛，这是最强烈的长期传播地点。新英格兰南部的田鼠至少包括两个主要种群，反对单一来源。南图克特集团包括玛莎葡萄园岛，南图克特和附近的科德角。康涅狄格/罗得岛（CT/RI）组包括来自这些州的所有样本沿着来自马萨诸塞州新兴地点的样本。新英格兰大陆南部的小孢子虫不是来自附近终碛岛（南图克特群）的寄生虫，而是来自CT/RI群。新B的开发。田鼠疫源地很可能是由于在整个新英格兰南部的残遗疫源地内传播的局部加强以及长距离传入事件的混合。
Background: Deer tick-transmitted human babesiosis due to Babesia microti appears to be expanding its distribution and prevalence in the northeastern United States. One hypothesis for this emergence is the introduction of parasites into new sites from areas of long-standing transmission, such as Nantucket Island, Massachusetts.Methods: We developed a typing system based on variable number tandem repeat loci that distinguished individual B. microti genotypes. We thereby analyzed the population structure of parasites from 11 sites, representing long-standing and newly emerging transmission in southern New England (northeastern United States), and compared their haplotypes and allele frequencies to determine the most probable number of B. microti populations represented by our enzootic collections. We expected to find evidence for a point source introduction across southern New England, with all parasites clearly derived from Nantucket, the site with the most intense longstanding transmission.Results: B. microti in southern New England comprises at least two major populations, arguing against a single source. The Nantucket group comprises Martha's Vineyard, Nantucket and nearby Cape Cod. The Connecticut/Rhode Island (CT/RI) group consists of all the samples from those states along with samples from emerging sites in Massachusetts.Conclusions: The expansion of B. microti in the southern New England mainland is not due to parasites from the nearby terminal moraine islands (Nantucket group), but rather from the CT/RI group. The development of new B. microti foci is likely due to a mix of local intensification of transmission within relict foci across southern New England as well as long distance introduction events.