Effect of angiotensin II on macrophage functions.
Effect of angiotensin II on macrophage functions.
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血管紧张素 II 对巨噬细胞功能的影响。
作者：
G. Fóris;B. Dezső;G. Medgyesi;G. Füst
血管紧张素 II (At II) 已被证明可以在体外抑制由 10(-5)-10(-6) M 浓度激发的腹膜巨噬细胞 (PM) 引起的 IgG2a 介导的 51Cr 羊红细胞 (SRBC) 玫瑰花结形成。玫瑰花结形成的减少与吞噬作用的增加有关。结果发现，在 10(-7) M 激素浓度下玫瑰花结形成增强，随后通过 Fc γ 受体 (R) 的吞噬作用减弱。仅在与 10(-5) M At II 一起孵育后，Fc microR 介导的过程才会受到影响。 At II 以剂量依赖性方式显着增强了通过 C3bR 的附着和随后的吞噬作用。因此，通过两个FcR的相对吞噬作用(RP)显着增强10(-5)，即At II的10(-5)-10(-6)M，但在10(-7)M激素浓度下降低。另外，At II与10(-5)M吲哚美辛(IM)预孵育后没有RP增强作用，表明前列腺素(PG)在激素作用中的重要性。含有 5 mM EGTA 的培养基减弱了 At II 的 RP 增强和抑制作用。 C3b 介导的 RP 不受 At II、IM 或 EGTA 的影响。通过白色念珠菌释放的铬来测量细胞内杀伤能力，在 At II 处理 PM 后没有改变，甚至略有减弱。
Angiotensin II (At II) has been shown to inhibit in vitro the IgG2a-mediated rosette formation of 51Cr-sheep red blood cells (SRBC) by provoked peritoneal macrophages (PM) at 10(-5)-10(-6) M concentrations. The decreased rosette formation was associated with an increased phagocytosis. It was found that the enhanced rosette formation at 10(-7) M hormone concentration was followed by diminished phagocytosis via Fc gamma receptors (R). Processes mediated through Fc microR were affected only after incubation with 10(-5) M of At II. The attachment and subsequent phagocytosis through C3bR was markedly enhanced by At II in a dose-dependent way. Thus, the relative phagocytosis (RP) through both FcRs was significantly enhanced by 10(-5), i.e. by 10(-5)-10(-6) M of At II, but lowered at 10(-7) M hormone concentration. In addition, there was no RP enhancing effect of At II after preincubation with 10(-5) M of indomethacin (IM), indicating the significance of prostaglandins (PG) in the hormone effect. The medium containing 5 mM of EGTA diminished both the RP enhancing and inhibiting effects of At II. The RP mediated by C3b was not affected by At II, IM or EGTA. The intracellular killing capability, measured by chromium release from Candida albicans, was not altered or even slightly diminished after At II treatment of PMs.