RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR STIMULATES INVITRO MATURE HUMAN NEUTROPHIL AND EOSINOPHIL FUNCTION, SURFACE-RECEPTOR EXPRESSION, AND SURVIVAL
RECOMBINANT HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR STIMULATES INVITRO MATURE HUMAN NEUTROPHIL AND EOSINOPHIL FUNCTION, SURFACE-RECEPTOR EXPRESSION, AND SURVIVAL
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DOI：
10.1172/jci112705
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发表时间：
1986-11-01
影响因子：
15.9
通讯作者：
VADAS, MA
中科院分区：
文献类型：
--
作者：
LOPEZ, AF;WILLIAMSON, DJ;VADAS, MA
纯化的重组人粒细胞-巨噬细胞集落刺激因子（rH GM-CSF）是一种强有力的成熟人嗜酸性粒细胞和中性粒细胞刺激因子。纯化的rH GM-CSF增强了中性粒细胞和嗜酸性粒细胞对抗体包被的靶点的细胞毒活性，以剂量依赖性方式刺激两种细胞类型对血清调理酵母的吞噬作用，并在酵母聚糖存在下刺激嗜中性粒细胞介导的碘化。此外，rH GM-CSF增强了N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）刺激的细胞松弛素B预处理的中性粒细胞的脱粒和FMLP刺激的超氧化物产生。与此相反，rH GM-CSF没有促进粒细胞粘附内皮细胞或塑料表面。rH GM-CSF选择性地增强粒细胞功能抗原1和2的表面表达，并且Mo 1抗原、rH GM-CSF诱导中性粒细胞和嗜酸性粒细胞的形态学改变，并分别增强中性粒细胞和嗜酸性粒细胞的存活6和9 h。这些实验表明，粒细胞-巨噬细胞集落刺激因子可以选择性地刺激成熟粒细胞功能。
A purified recombinant human granulocyte-macrophage colony stimulating factor (rH GM-CSF) was a powerful stimulator of mature human eosinophils and neutrophils. The purified rH GM-CSF enhanced the cytotoxic activity of neutrophils and eosinophils against antibody-coated targets, stimulated phagocytosis of serum-opsonized yeast by both cell types in a dose-dependent manner, and stimulated neutrophil-mediated iodination in the presence of zymosan. In addition, rH GM-CSF enhanced N-formylmethionylleucylphenylalanine (FMLP)-stimulated degranulation of Cytochalasin B pretreated neutrophils and FMLP-stimulated superoxide production. In contrast, rH GM-CSF did not promote adherence of granulocytes to endothelial cells or plastic surfaces. rH GM-CSF selectively enhanced the surface expression of granulocyte functional antigens 1 and 2, and the Mo1 antigen, rH GM-CSF induced morphological changes and enhanced the survival of both neutrophils and eosinophils by 6 and 9 h, respectively. These experiments show that granulocyte-macrophage colony stimulating factor can selectively stimulate mature granulocyte function.