Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.
Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.
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DOI：
10.1038/ncomms5509
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发表时间：
2014-07-25
影响因子：
16.6
通讯作者：
Rizzoli SO
中科院分区：
文献类型：
--
作者：
Saka SK;Honigmann A;Eggeling C;Hell SW;Lang T;Rizzoli SO
大多数蛋白质在质膜上分布不均匀。一般来说，这可能是由每种蛋白质特有的机制引起的，也可能是影响所有膜蛋白分布的一般模式的结果。后一种假设在过去很难检验。在这里，我们介绍了几种基于点击化学的方法，通过这些方法我们研究了活细胞以及膜片中膜蛋白的分布。我们发现，质膜蛋白形成多蛋白组装体，其寿命长（分钟），并且其中蛋白质扩散受到限制。组装体的形成依赖于胆固醇。它们被肌动蛋白细胞骨架分开并锚定。特定的蛋白质优先位于组件的不同区域，从它们的核心到它们的边缘。我们的结论是，组件构成了一个基本的介观特征的膜，这会影响大多数膜蛋白的图案，也可能是他们的活动。 虽然许多蛋白质在质膜中采用不均匀分布，但尚不清楚这些纳米级异质性如何与膜的一般蛋白质图案化相关。Saka等人使用点击化学来揭示膜蛋白到多蛋白组装体中的中尺度组织。
Most proteins have uneven distributions in the plasma membrane. Broadly speaking, this may be caused by mechanisms specific to each protein, or may be a consequence of a general pattern that affects the distribution of all membrane proteins. The latter hypothesis has been difficult to test in the past. Here, we introduce several approaches based on click chemistry, through which we study the distribution of membrane proteins in living cells, as well as in membrane sheets. We found that the plasma membrane proteins form multi-protein assemblies that are long lived (minutes), and in which protein diffusion is restricted. The formation of the assemblies is dependent on cholesterol. They are separated and anchored by the actin cytoskeleton. Specific proteins are preferentially located in different regions of the assemblies, from their cores to their edges. We conclude that the assemblies constitute a basic mesoscale feature of the membrane, which affects the patterning of most membrane proteins, and possibly also their activity. Although many proteins adopt uneven distributions in the plasma membrane, it is not clear how these nanoscale heterogeneities relate to the general protein patterning of the membrane. Saka et al. use click chemistry to reveal the mesoscale organization of membrane proteins into multi-protein assemblies.
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DOI：
10.1083/jcb.81.2.275
发表时间：
1979-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heuser JE;Reese TS;Dennis MJ;Jan Y;Jan L;Evans L
通讯作者：
Evans L
影响因子：
3.4
作者：
Honigmann, Alf;Mueller, Veronika;Eggeling, Christian
通讯作者：
Eggeling, Christian
影响因子：
3.4
作者：
de Meyer, Frederick J. M.;Rodgers, Jocelyn M.;Smit, Berend
通讯作者：
Smit, Berend
影响因子：
3.4
作者：
Frankel, DJ;Pfeiffer, JR;Burns, AR
通讯作者：
Burns, AR
影响因子：
4
作者：
Cambi, Alessandra;Lidke, Diane S.
通讯作者：
Lidke, Diane S.