Molecular characterization of differentially expressed TXNIP gene and its association with porcine carcass traits
Molecular characterization of differentially expressed TXNIP gene and its association with porcine carcass traits
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差异表达TXNIP基因的分子特征及其与猪胴体性状的关联
DOI：
10.1007/s11033-012-1923-2
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Xu, Dequan
中科院分区：
文献类型：
--
作者：
Liu, Min;Ling, Xianfeng;Xu, Dequan
硫氧还蛋白相互作用蛋白（TXNIP）在长白猪×约克郡杂种F1代骨骼肌中表达下调，在脂质代谢和免疫调节中起重要作用。本文对猪TXNIP基因的分子生物学特性进行了描述。该cDNA全长编码1,176 bp，5 '端非翻译区263 bp，3'端非翻译区441 bp。预测的猪TXNIP的分子量和等电点分别为43.81 kDa和7.385。推导的391个氨基酸与其他哺乳动物TXNIP具有高度的同源性。TXNIP基因由8个编码外显子和7个非编码内含子组成，全长约3, 348 bp。在骨骼肌发育过程中，长×约克郡猪TXNIP mRNA几乎不表达，6月龄猪表达水平较低。其它阶段和品种的表达量较高。统计分析表明，TXNIP基因多态性（c.575- 4 T> C）在约克郡×梅山F2群体中与亲本间存在差异，与屠宰率（DP）和胸腰脂肪厚度（TFT）高度相关。并对TXNIP的可能作用进行了讨论。
Thioredoxin interacting protein (TXNIP), which plays a regulatory role in lipid metabolism and immune regulation, is down-regulated expressed in F-1 hybrids Landrace x Yorkshire skeletal muscle. Here we described the molecular characterization of porcine TXNIP gene. The full-length cDNA contains a coding sequence of 1,176 bp nucleotides with untranslated regions of 263 bp at 5'-end and 441 bp at 3'-end, respectively. The predicted molecular mass and isoelectric point of porcine TXNIP is 43.81 kDa and 7.385, respectively. The deduced 391 amino acids exhibit high identity with other mammalian TXNIP. The TXNIP gene contains eight coding exons and seven non coding introns, spans approximately 3,348 bp. The expression of porcine TXNIP mRNA is almost absent in Landrace x Yorkshire and lower level in 6-month-old pigs during skeletal muscle development. Other stages and breeds were high level expressed. Statistical analysis showed the TXNIP gene polymorphism (c.575-4T > C) was different between F-1 hybrids and their parents, was highly associated with dressing percentage (DP) and thorax-waist fat thickness (TFT) in the Yorkshire x Meishan F-2 population. The possible role of TXNIP was discussed.