A Study of ΔNp63 Expression in Lung Non-Small Cell Carcinomas
A Study of ΔNp63 Expression in Lung Non-Small Cell Carcinomas
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DOI：
10.1097/pas.0b013e3182498f2b
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发表时间：
2012-06-01
影响因子：
5.6
通讯作者：
Nonaka, Daisuke
中科院分区：
文献类型：
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作者：
Nonaka, Daisuke
鉴于不同的治疗方案，区分肺腺癌和鳞状细胞癌变得越来越重要。虽然两者之间的组织学细分在分化肿瘤中通常并不困难，但在低分化肿瘤中可能具有挑战性，可能需要一组免疫组织化学染色。p63基因编码两个不同的N-末端（TA和Δ N）。Delta Np 63选择性地在鳞状细胞癌中表达，而TAp 63不仅限于此。4A 4是一种广泛使用的抗p63抗体，可识别两种亚型，并在约15%的腺癌中表达，尽管通常是局灶性的，但其表达可以是弥漫性的。在这项研究中，共150例肺腺癌和50例鳞状细胞癌的p63（4A 4），Δ Np 63（p40），和TTF-1（8 G7 G3/1）的抗体免疫染色。27例腺癌（18%）呈不同程度的p63阳性，13例肿瘤（8.7%）呈弥漫性反应。p63表达见于除粘液型外的所有腺癌亚型。所有腺癌中p40均为阴性。所有鳞状细胞癌均为p63和p40弥漫阳性。27例p63阳性腺癌中有4例TTF-1阴性。p63表达在腺癌中并不少见，而Delta Np 63（p40）表达对鳞状细胞癌具有特异性，其敏感性与p63表达相当。低分化肺腺癌中p63阳性细胞的存在可能被错误地解释为鳞状细胞分化的证据。p40似乎是鳞状细胞癌更可靠的标志物。
Distinguishing between lung adenocarcinoma and squamous cell carcinoma is becoming increasingly important, given the different treatment regimens available. Although histologic subdivision between the two is generally not difficult in differentiated tumors, it can be challenging in poorly differentiated tumors and may require a panel of immunohistochemistry stains. The p63 gene encodes two different N-termini (TA and Delta N). Delta Np63 is selectively expressed in squamous cell carcinoma, whereas TAp63 is not restricted only to it. 4A4, a widely used anti-p63 antibody, identifies both isoforms and is expressed in about 15% of adenocarcinomas, and, although generally focal, its expression can be diffuse. In this study, a total of 150 lung adenocarcinomas and 50 squamous cell carcinomas were immunostained by antibodies for p63 (4A4), Delta Np63 (p40), and TTF-1 (8G7G3/1). Twenty-seven adenocarcinomas (18%) were positive for p63 to a variable extent, with diffuse reaction being seen in 13 tumors (8.7%). p63 expression was seen in all subtypes of adenocarcinomas, except for the mucinous type. p40 was negative in all adenocarcinomas. All squamous cell carcinomas were diffusely positive for both p63 and p40. Four of 27 p63-positive adenocarcinomas were negative for TTF-1. p63 expression is not uncommonly seen in adenocarcinomas, whereas Delta Np63 (p40) expression is specific for squamous cell carcinoma, with sensitivity comparable to that of p63 expression. Presence of p63-positive cells in poorly differentiated lung adenocarcinoma may be erroneously interpreted as evidence of squamous cell differentiation. p40 appears to be a more reliable marker for squamous cell carcinoma.