Detection of serum-hepatitis antigen by charcoal particle agglutination-inhibition.
Detection of serum-hepatitis antigen by charcoal particle agglutination-inhibition.
复制标题
通过木炭颗粒凝集抑制检测血清肝炎抗原。
作者：
R. Stevens;F. D. Ziegler;J. H. Kelly
建立了一种检测和定量检测血清肝炎相关抗原的新方法。这种抗原，除了与血清肝炎(1，2)有关外，最近还与其他多种疾病状态有关(3-5)。检测试剂为吸附在颗粒炭上的血清肝炎(SH)抗原。这些颗粒-抗原复合体由同源抗血清宏观聚集，通过血清样本抑制这种凝集形成抗原测试。本函件描述了该方法并报告了血清学评估的结果。
材料和方法血清肝炎抗原(批号28-56-1)经区带超速离心法纯化，由美国马里兰州贝塞斯达的Electro-Nuconics实验室公司提供。20014。在对0.85%生理盐水进行透析后，将抗原稀释并与细小木炭颗粒的水悬浮液混合(Hynson，Westcott和Dunning，Inc.，巴尔的摩，马里兰州)。21201)。
A new method has been developed for the detection and quantitation of the antigen related to serum hepatitis. This antigen, in addition to its association with serum hepatitis (1, 2), has been implicated more recently in a variety of other disease states (3–5). The test reagent is serum-hepatitis (SH) antigen adsorbed to particulate charcoal. These particle-antigen complexes are macroscopically aggregated by homologous antisera and inhibition of this agglutination by a serum specimen forms the test for antigen. This communication describes the method and reports the findings of a serologic evaluation.
Materials and Methods. Serum-hepatitis antigen (lot 28-56-1) purified by zonal ultracentrifugation was obtained from Electro-Nucleonics Laboratories, Inc., Bethesda, Md. 20014. The antigen, after dialysis against 0.85% saline, was diluted and mixed with an aqueous suspension of fine charcoal particles (Hynson, Westcott, and Dunning, Inc., Baltimore, Md. 21201).