Analyses of peripheral blood mononuclear cells in operational tolerance after pediatric living donor liver transplantation
Analyses of peripheral blood mononuclear cells in operational tolerance after pediatric living donor liver transplantation
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DOI：
10.1111/j.1600-6143.2004.00611.x
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发表时间：
2004-12-01
影响因子：
8.8
通讯作者：
Tanaka, K
中科院分区：
文献类型：
--
作者：
Li, Y;Koshiba, T;Tanaka, K
我们的选择性方案可使部分患者在活体肝移植（LDLT）后出现手术耐受（在无免疫抑制（IS）的环境中接受移植物）。然而，没有可靠的参数来监测可能停止IS而没有排斥风险的患者。为了确定这些参数，我们对手术耐受患者的外周血单个核细胞进行了系统表型分析。与年龄匹配的志愿者（Gr-vol; n = 24）或接受IS治疗的患者（Gr-IS; n = 19）相比，在手术耐受患者（Gr-tol; n = 12）中观察到CD 4 + CD 25（高+）细胞、B细胞和V δ 1/V δ 2 γ δ T细胞比率的频率增加。NK细胞的频率下降，在Gr-tol，与那些在Gr-IS或Gr-卷。LDLT后的NKT细胞的频率下降，与Gr-卷。虽然这些子集的耐受状态的贡献仍然难以捉摸，结果可能提供重要的线索，LDLT后的耐受性的可靠指标。
Operational tolerance (graft acceptance in an immunosuppression (IS)-free environment) after living-donor liver transplantation (LDLT) could occur by our elective protocol in some patients. There is, nevertheless, no reliable parameter to monitor patients who may discontinue IS without a risk of rejection. To identify such parameters, we systemically phenotyped peripheral blood mononuclear cells from operationally tolerant patients. An increase was observed in the frequency of CD4 + CD25(high+) cells, B cells and Vdelta1/Vdelta2 gammadelta T-cells ratio in operationally tolerant patients (Gr-tol; n = 12), compared with those from age-matched volunteers (Gr-vol; n = 24) or patients on IS (Gr-IS; n = 19). The frequency of NK cells was decreased in Gr-tol, compared with those in Gr-IS or Gr-vol. The frequency of NKT cells was decreased after LDLT, compared with that in Gr-vol. Although the contribution of those subsets to the tolerant state remains elusive, the results may provide important clues for reliable indicators of tolerance after LDLT.