Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium
Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium
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DOI：
10.1046/j.1365-313x.2000.00757.x
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发表时间：
2000-06-01
期刊：
影响因子：
7.2
通讯作者：
Harrison, MJ
中科院分区：
文献类型：
--
作者：
Trieu, AT;Burleigh, SH;Harrison, MJ
已经为模式豆科蒺藜苜蓿开发了两种快速且简单的植物转化方法。第一种方法基于为拟南芥转化而开发的方法，涉及用农杆菌悬浮液渗透开花植物。第二种方法涉及用农杆菌渗透幼苗。在这两种情况下，渗透植物的后代的一部分被转化。浸花法的转化频率为4.7%～76%，浸苗法的转化频率为2.9%～27.6%。两种方法均产生独立转化体和同级转化体的混合物。转化子在遗传上是稳定的，对T-2代的分析表明转基因以孟德尔方式遗传。这些转化系统将提高蒺藜 M. truncatula 作为模型系统的效用，并实现大规模插入诱变。 T-DNA 标记和该方法的许多改进为分析豆类的独特方面提供了广泛的机会。
Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T-2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.