Effects of various single-stranded-DNA-binding proteins on reactions promoted by RecA protein.
Effects of various single-stranded-DNA-binding proteins on reactions promoted by RecA protein.
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各种单链 DNA 结合蛋白对 RecA 蛋白促进的反应的影响。
DOI：
10.1128/jb.169.8.3422-3428.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Chase,JW
中科院分区：
文献类型：
--
作者：
Egner,C;Azhderian,E;Tsang,SS;Radding,CM;Chase,JW
为了了解大肠杆菌 SSB 在体内和体外重组中的作用，我们研究了突变蛋白 SSB-1 和 SSB-113、胰凝乳蛋白酶裂解产生的变体 SSBc、部分同源变体 F SSB（由大肠杆菌性因子编码）以及噬菌体 T4 基因 32 编码的蛋白。除 SSB-1 外，所有这些都增强了 RecA 蛋白促进的同源配对和链交换的初始速率。从这些和相关观察中，我们得出结论，SSB 通过非特异性促进 RecA 蛋白与单链 DNA 的结合来刺激接头分子的初始形成； SSB 在 RecA 核蛋白丝与双链 DNA 的突触中不起任何作用； SSB 对链交换的刺激同样是非特异性的；以 SSB、F SSB 和基因 32 蛋白为代表的蛋白类的所有成员可能在使单链 DNA 更容易接近 RecA 蛋白方面发挥相同的作用。
To relate the roles of Escherichia coli SSB in recombination in vivo and in vitro, we have studied the mutant proteins SSB-1 and SSB-113, the variant SSBc produced by chymotryptic cleavage, the partially homologous variant F SSB (encoded by the E. coli sex factor), and the protein encoded by gene 32 of bacteriophage T4. All of these, with the exception of SSB-1, augmented both the initial rate of homologous pairing and strand exchange promoted by RecA protein. From these and related observations, we conclude that SSB stimulates the initial formation of joint molecules by nonspecifically promoting the binding of RecA protein to single-stranded DNA; that SSB plays no role in synapsis of the RecA nucleoprotein filament with duplex DNA; that stimulation of strand exchange by SSB is similarly nonspecific; and that all members of the class of proteins represented by SSB, F SSB, and gene 32 protein may play equivalent roles in making single-stranded DNA more accessible to RecA protein.