EXPRESSION OF MESSENGER-RIBONUCLEIC-ACID SPECIES ENCODING STEROIDOGENIC ENZYMES IN HUMAN FOLLICLES AND CORPORA-LUTEA THROUGHOUT THE MENSTRUAL-CYCLE
EXPRESSION OF MESSENGER-RIBONUCLEIC-ACID SPECIES ENCODING STEROIDOGENIC ENZYMES IN HUMAN FOLLICLES AND CORPORA-LUTEA THROUGHOUT THE MENSTRUAL-CYCLE
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DOI：
10.1210/jcem-70-4-1041
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发表时间：
1990-04-01
影响因子：
5.8
通讯作者：
SIMPSON, ER
中科院分区：
文献类型：
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作者：
DOODY, KJ;LORENCE, MC;SIMPSON, ER
编码胆固醇侧链裂解细胞色素P-450（450 SCC），17 α-β-葡聚糖的mRNA种类的表达水平羟化酶细胞色素P450（P45017 α），芳香酶细胞色素P-450（P-450 AROM）和3 β-在整个月经周期中检测人卵泡和黄体（CL）中的羟类固醇脱氢酶（3 β HSD）。组织取自行子宫切除术和卵巢切除术的妇女。根据手术是在卵泡期还是黄体期进行，从卵巢中切除最大的卵泡或CL。周期的日期由末次月经的开始确定，并由子宫内膜组织学证实。通过北方印迹分析，使用编码每种人酶的特异性32 P标记的cDNA插入片段作为探针，检查总RNA。早期卵泡显示出P450 SCC和P450 P45017 α的可检测mRNA，但对P450 AROM或3 β HSD则不适用。P450 AROM在卵泡晚期表达，在CL中出现明显诱导表达。3.仅在CL中检测到β. HSD。P45017.alpha的水平。mRNA在整个周期中保持相对不变，而P450 SCC mRNA水平在CL中大大增加。P45017 α的存在。人CL中的mRNA是令人感兴趣的，因为它不存在于牛CL中，并且这与人CL而不是牛CL合成17 α的能力一致。羟孕酮和雌激素。P450 AROM表达在CL中最高的事实是令人惊讶的，因为血浆雌激素水平在周期的晚期卵泡期最高，并且可能表明CL雌激素生物合成受到17 α限制。羟化酶或17，20-裂解酶活性。
The levels of expression of mRNA species encoding cholesterol side-chain cleavage cytochrome P-450 (450scc), 17.alpha.-hydroxylase cytochrome P450 (P45017.alpha.), aromatase cytochrome P-450 (P-450AROM), and 3.beta.-hydroxysteroid dehydrogenase (3.beta.HSD) were examined in human follicles and corpora lutea (CL) throughout the menstrual cycle. Tissues were obtained from women undergoing hysterectomy and oophorectomy. The largest follicle or the CL was dissected from the ovary depending on whether the surgery was performed in the follicular or luteal phase. The day of the cycle was determined by onset of last menstrual period and was confirmed by endometrial histology. Total RNA was examined by Northern blot analysis, using as probes specific 32P-labeled cDNA inserts encoding each human enzyme. Early follicles demonstrated detectable mRNA for both P450scc and P450P45017.alpha., but not for P450AROM or 3.beta.HSD. P450AROM was detectable late in the follicular phase and appeared markedly induce din the CL. 3.BETA.HSD was detected only in the CL. Levels of P45017.alpha. mRNA remained relatively unchanged throughout the cycle, whereas P450scc mRNA levels were greatly increased in the CL. The presence of P45017.alpha. mRNA in the human CL is of interest, since it is absent from the bovine CL, and this is consistent with the ability of the human, but not the bovine, CL to synthesize 17.alpha.-hydroxyprogesterone and estrogens. The fact that P450AROM expression is highest in CL is surprising, since plasma estrogen levels are highest during the late follicular phase of the cycle, and may suggest that CL estrogen biosynthesis is limited by 17.alpha.-hydroxylase or 17,20-lyase activities.