SUPPRESSION OF MURINE NATURAL-KILLER-CELL ACTIVITY BY TRIBUTYLTIN - INVIVO AND INVITRO ASSESSMENT
SUPPRESSION OF MURINE NATURAL-KILLER-CELL ACTIVITY BY TRIBUTYLTIN - INVIVO AND INVITRO ASSESSMENT
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DOI：
10.1016/s0013-9351(05)80252-x
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发表时间：
1990-08-01
影响因子：
8.3
通讯作者：
ALFRED, LJ
中科院分区：
文献类型：
--
作者：
GHONEUM, M;HUSSEIN, AE;ALFRED, LJ
本文研究了氯化三丁基锡（TBTCl）和无机锡（IT）对小鼠自然杀伤（NK）细胞活性的影响。体内研究表明，与对照小鼠相比，在效应物：靶（E：T）比= 50：1时，每天喂食三丁基锡化合物（10和100 ppm）一周的小鼠表现出NK活性抑制38-46%。另一方面，用无机锡处理的动物的NK细胞活性没有变化。体外研究表明，白细胞，预孵育TBTCl（0.01-0.1 ppm）在室温下1小时，然后洗涤三次，表现出显着的抑制NK活性（41和85%）在浓度0.01和0.05 ppm，分别。将剂量增加至0.1 ppm，导致NK细胞活性的完全抑制。相反，在相同浓度的TBTCl下，IT对体外NK活性没有影响。TBTCl的作用似乎是由于干扰效应细胞的结合能力，这是靶细胞裂解的必要先决条件。总之，TBTCl被证明是一种非常有效的NK活性抑制剂，这种抑制作用可能使动物易患恶性肿瘤，这是最近报道的一些TBT化合物的特征。
The effects of tributyltin chloride (TBTCl) and inorganic tin (IT) on murine natural killer (NK) cell activity were tested in vivo and in vitro. In vivo studies demonstrated that mice fed with TBT (10 and 100 ppm) daily for 1 week exhibited suppression in NK activity 38-46% at effector:target (E:T) ratio = 50:1 compared to control mice. On the other hand, animals treated with inorganic tin showed no change in activity of NK cells. In vitro studies showed leukocytes, preincubated with TBTCl (0.01-0.1 ppm) at room temperature for 1 h and then washed three times, demonstrated significant suppression in NK activity (41 and 85%) at concentrations 0.01 and 0.05 ppm, respectively. Increasing the dose to 0.1 ppm, resulted in complete inhibition of the activity of NK cells. In contrast, IT had no effect on NK activity in vitro at the same concentrations of TBTCl. The effect of TBTCl appears to be due to interference with the binding capacity of effector cells, a necessary prerequisite for target cell lysis. In conclusion, TBTCl proved to be a very potent inhibitor of NK activity; this inhibition may predispose animals to malignancy, which is a characteristic feature reported recently for some TBT compounds.