Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing
Heterologous Expression and Characterization of a Ferulic Acid Esterase from Aspergillus aculeatus with Potential Use in Sunflower Seed Processing
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棘孢曲霉阿魏酸酯酶的异源表达和表征及其在葵花籽加工中的潜在应用
DOI：
10.3390/app11104453
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Juncai Leng
中科院分区：
文献类型：
--
作者：
Wenbin Zhang;Jieyu Wang;Yuechen Liu;Juncai Leng
绿原酸（CGA）水解活性是阿魏酸酯酶的重要副作用。为提高阿魏酸酯酶（FAE）的表达效率，探索其在葵花籽加工中的应用。从棘孢曲霉（Aspergillusaculeatus）中获得两个新的FAE（AaSD 14），并在基因工程菌E. coliBL 21（DE 3），并对其进行了温度、pH、金属离子和底物特异性等性质的表征。重组阿魏酸酯酶（reFAE）具有竞争性水解CGA的活性，reFAE 1和reFAE 2分别为246.37 U/g和340.95 U/g，分别是野生型（4.35 U/g）的56.6和78.4倍。同时，发酵周期大大缩短至2.0d。通过底物特异性分析，这些reFAE被识别为C型FAE。用reFAE 2处理向日葵种子蛋白（SSP）导致显著的颜色变化，从绿色变为乳白色，证实了CGA生物降解的活性。因此，在葵花籽及相关食品的加工中显示出一定的潜力。
: Hydrolytic activity for chlorogenic acid (CGA) has been recognized as an important side activity of some types of ferulic acid esterases. The purpose of this work was to enhance the efficient expression of ferulic acid esterase (FAE) and to explore its application in the processing of sunflower seed. Two novel FAEs from Aspergillus aculeatus ( Aa SD14) were expressed in genetically engineered E. coli BL21 (DE3), and their properties, including temperature, pH, metal ions and substrate specificity, were characterized after purification. Competitive CGA hydrolysis activity was observed in these recombined ferulic acid esterases (reFAEs) with reFAE1 of 246.37 U/g and reFAE2 of 340.95 U/g, which were 56.6 and 78.4 times higher than that of the wild strain (4.35 U/g), respectively. Meanwhile, the fermentation cycle was greatly shortened to 2.0 d. These reFAEs were recognized as type C FAE through substrate specificity assays. Treatment of sunflower seed protein (SSP) using reFAE2 resulted in a remarkable color change, from green to milk-white, confirming the activity of CGA biodegradation. Therefore, it shows certain potential in the processing of sunflower seed and other related foodstuffs.
影响因子：
4.4
作者：
Nieter, Annabel;Haase-Aschoff, Paul;Berger, Ralf G.
通讯作者：
Berger, Ralf G.