Detection of plant genes, gene expression and viral RNA from tissue prints on FTA cards
Detection of plant genes, gene expression and viral RNA from tissue prints on FTA cards
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从 FTA 卡上的组织印迹中检测植物基因、基因表达和病毒 RNA
DOI：
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发表时间：
2005
影响因子：
2.1
通讯作者：
A. Nassuth
中科院分区：
文献类型：
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作者：
Yvette Roy;A. Nassuth
建立了一种用于容易和方便地检测来自FTA®上的植物组织印迹的植物DNA、植物RNA和病毒RNA的程序（Flinders Technology Associates，Moscoso等人，2005年）PlantSaver卡，同时避免交叉污染，通常发生与打印从植物组织。通过将2 mm的印品圆盘直接加入（RT）-PCR反应中，检测成功。在烟草、番茄和葡萄的叶片以及番茄和葡萄的果实中检测到DNA。在番茄果实和叶片、烟草叶片和葡萄叶片中检测到Rubisco和苹果酸脱氢酶RNA。在番茄叶片和果实中检测到来自佩皮诺花叶病毒的RNA，在葡萄叶片中检测到来自鲁佩斯蒂斯茎痘相关病毒的RNA。在室温下储存数月后，仍然可以从FTA®卡上的组织印迹中进行检测，这使得该程序适用于在实验室进一步处理之前的样本采集和场外储存。
A procedure was established for easy and convenient detection of plant DNA, plant RNA and viral RNA from plant tissue prints on FTA® (Flinders Technology Associates, Moscoso et al., 2005) PlantSaver Cards, while avoiding the cross-contamination that commonly occurs with prints from plant tissues. Detection was successful by adding 2 mm discs of the prints directly to (RT)-PCR reactions. DNA was detected in leaves from tobacco, tomato and grapes, and in fruit of tomato and grape. Rubisco and malate dehydro-genase RNA were detected in tomato fruit and leaf, tobacco leaf and grape leaf. RNA from Pepino mosaic virus was detected in tomato leaf and fruit and from Rupestis stem pitting associated virus in grape leaf. Detection was still possible from the tissue prints on FTA® Cards after storage for many months at room temperature, making this a procedure suitable for sample collection and storage off site, prior to further processing in the laboratory.