Notch mediates the segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos
Notch mediates the segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos
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DOI：
10.1016/j.devcel.2008.03.024
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发表时间：
2008-06-01
影响因子：
11.8
通讯作者：
Takahashi, Yoshiko
中科院分区：
文献类型：
--
作者：
Sato, Yuki;Watanabe, Tadayoshi;Takahashi, Yoshiko
我们研究了，使用鸟类胚胎，背后的机制的背主动脉，第一次形成胚胎血管在Aubrootes的三维组装。该导管起源于两个不同的细胞群体，内脏和体节中胚层。我们已经揭示了Notch信号在体细胞贡献中的作用。在激活Notch信号传导后，个体体节后半部分的细胞亚群向腹侧向内脏起源的初级背主动脉迁移。在到达主动脉后，这些体节细胞分化为永久性主动脉内皮细胞。这种Notch诱导的腹侧迁移是由EphrinB2和初级主动脉的引诱作用介导的。此外，通过转座子介导的基因转移对细胞的长期追逐揭示了背主动脉中节段性提供的体节起源的内皮细胞最终填充血管的整个区域。我们展示了从间充质细胞形成胚胎血管的分子和细胞机制。
We studied, using avian embryos, mechanisms underlying the three-dimensional assembly of the dorsal aorta, the first-forming embryonic vessel in amniotes. This vessel originates from two distinct cell populations, the splanchnic and somitic mesoderms. We have unveiled a role for Notch signaling in the somitic contribution. Upon activation of Notch signaling, a subpopulation of cells in the posterior half of individual somites migrates ventrally toward the primary dorsal aorta of splanchnic origin. After reaching the primary aorta, these somitic cells differentiate into the definitive aortic endothelial cells. This Notch-induced ventral migration is mediated by EphrinB2 and by an attractant action of the primary aorta. Furthermore, long-term chasing of cells by transposon-mediated gene transfer reveals that the segmentally provided endothelial cells of somitic origin in the dorsal aorta ultimately populate the entire region of the vessel. We demonstrate the molecular and cellular mechanisms underlying the formation of embryonic blood vessels from mesenchymal cells.