TT virus infection in nonhuman primates and characterization of the viral genome: Identification of simian TT virus isolates
TT virus infection in nonhuman primates and characterization of the viral genome: Identification of simian TT virus isolates
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DOI：
10.1128/jvi.74.3.1549-1553.2000
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发表时间：
2000-02-01
影响因子：
5.4
通讯作者：
Goto, S
中科院分区：
文献类型：
--
作者：
Abe, K;Inami, T;Goto, S
新发现的TT病毒（TTV）在人群中广泛分布。为了进一步了解ITV与宿主的关系，我们用PCR方法检测了400份不同灵长类动物血清中TTV DNA的存在。我们从24种不同的非人灵长类动物中收集了血清样本。TTV DNA通过PCR确定，所述PCR使用从TTV基因组的5 '端区域设计的引物。还进行了病毒基因组的核苷酸测序和系统发育分析。在98只黑猩猩中的87只（89%）和21只食蟹猕猴中的3只（14%）中检测到TTV DNA。从两只动物中获得的PCR产物的核苷酸序列在两个物种之间具有80%至100%的相同性。相比之下，该序列与人类TTV分离株在核苷酸水平上的差异为24%至33%，在氨基酸水平上的差异为36%至50%。系统发育分析表明，所有从猴中分离的TTV均与人TTV分离株不同。此外，TTV在猿，而不是在人类，被分为三个不同的基因型。我们的研究结果表明，TTV在猿猴代表一组不同，但密切相关，TTV在人类。根据这些结果，我们暂时将这种TTV命名为猿猴TTV（s-TTV）。s-TTV的存在对于确定人类TTV的起源、性质和传播具有重要意义，并可能为研究这种新的DNA病毒的感染和发病机制提供有用的动物模型。
Newly discovered TT virus (TTV) is widely distributed in human populations. To understand more about the relationship between ITV and its hosts, we tested 400 sera from various nonhuman primates for the presence of TTV DNA by PCR assay. We collected serum samples from 24 different species of nonhuman primates. TTV DNA,vas determined by PCR with primers designed from the 5'-end region of the TTV genome. Nucleotide sequencing and phylogenetic analysis of viral genomes were also performed. TTV DNA was detected in 87 of 98 (89%) chimpanzees and 3 of 21 (14%) crab-eating macaques. Nucleotide sequences of the PCR products obtained from both animals were 80 to 100% identical between two species. In contrast, the sequences differed from TTV isolates in humans by 24 to 33% at the nucleotide level and 36 to 50% at the amino acid level. Phylogenetic analysis demonstrated that all TTV isolates obtained From simians were distinct from the human TTV isolates. Furthermore, TTV in simians, but not in humans, was classified into three different genotypes. Our results indicate that TTV in simians represents a group different from, but closely related to, TTV in humans. From these results, we tentatively named this TTV simian TTV (s-TTV). The existence of the s-TTV will be important in determining the origin, nature, and transmission of human TTV and may provide useful animal models for studies of the infection and pathogenesis of this new DNA virus.