Preparation and characterization of a gallium-loaded antimicrobial artificial dermal scaffold
Preparation and characterization of a gallium-loaded antimicrobial artificial dermal scaffold
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载镓抗菌人工真皮支架的制备及表征
DOI：
10.1016/j.msec.2019.110063
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Xia Zhaofan
中科院分区：
文献类型：
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作者：
Xu Zhaorong;Chen Xiaodong;Tan Rongwei;She Zhending;Chen Zhaohong;Xia Zhaofan
人工真皮支架由天然或合成材料制成，植入创面后能促进新血管形成、细胞迁移和细胞增殖，降解缓慢，在真皮重建和瘢痕抑制方面发挥重要作用，最终达到创面愈合和功能重建的目的。尽管这些支架已广泛应用于临床，但生物材料相关感染是一个必须解决的缺陷甚至是威胁生命的问题，因为它极大地影响了支架的存活。镓离子（Ga 3+）是一种新型金属抗菌剂，其对烧伤伤口感染中遇到的大多数细菌的广谱抗菌特性已被证实，并且已被提出作为预防植入物相关感染的有希望的候选物。本研究以镓离子和胶原蛋白溶液为原料，成功制备了载镓抗菌人工真皮支架。表征结果表明，该材料具有多孔结构，孔径范围为50 ~ 150 μm，孔隙率高达97.4%。12 h和24 h的体外酶降解率分别为19%和28%，体外抑菌试验表明，1h对金黄色葡萄球菌和铜绿假单胞菌的抑菌率接近90%，具有较强的抗菌活性。细胞学评价结果显示，对细胞增殖的影响轻微，相对生长率（RGR）值为80%，根据激光扫描共聚焦显微镜（LSCM）和扫描电子显微镜（SEM）与培养的细胞具有良好的细胞相容性。此外，通过体内抗菌评价证实了人工真皮支架成功地预防了SD大鼠伤口感染，并且人工真皮支架也表现出良好的生物相容性。该载镓抗菌人工真皮支架具有良好的抗菌活性和生物安全性，值得进一步研究。
Artificial dermal scaffolds, which are made of natural or synthetic materials, can improve new blood vessel formation, cell migration and cell proliferation after being implanted into wounds, and they degrade slowly, playing an important role in dermal reconstruction and scar inhibition, finally achieving the goal of wound healing and functional reconstruction. Although these scaffolds have been widely used in clinical applications, biomaterial-associated infection is a deficiency or even a life-threatening problem that must be addressed, as it greatly affects the survival of the scaffolds. The gallium ion (Ga3+) is a novel metallic antimicrobial whose broad-spectrum antimicrobial properties against most bacteria encountered in burn wound infections have been confirmed, and it has been proposed as a promising candidate to prevent implant-associated infections. In this study, a gallium-loaded antimicrobial artificial dermal scaffold was successfully prepared by gallium ions and a collagen solution. The characterization results showed a porous structure with pore sizes ranging from 50 to 150 μm and a large porosity value of 97.4%. The enzymatic degradation ratein vitrowas 19 and 28% after 12 and 24 h, respectively.In vitroantimicrobial testing revealed that the 1 h antibacterial rate against Staphylococcus aureus and Pseudomonas aeruginosa was close to 90%, which indicated its great antimicrobial activity. The results of the cytological evaluation showed slight effect on cell proliferation, with a relative growth rate (RGR) value of 80% and great cytocompatibility with cultured cells according to laser scanning confocal microscopy (LSCM) and scanning electron microscope (SEM). Furthermore, the successful prevention of wound infections in SD rats was confirmed with anin vivoantimicrobial evaluation, and the artificial dermal scaffolds also demonstrated great biocompatibility. This gallium-loaded antimicrobial artificial dermal scaffold exerted excellent antimicrobial activity and great biosafety, warranting further research for future clinical applications.