Membrane-reinforced three-dimensional electrospun silk fibroin scaffolds for bone tissue engineering
Membrane-reinforced three-dimensional electrospun silk fibroin scaffolds for bone tissue engineering
复制标题
DOI：
10.1088/1748-6041/10/3/035011
复制
发表时间：
2015-06
影响因子：
4
通讯作者：
Sungyeun Yang;T. Hwang;Lihua Che;J. Oh;Yoon Ha;W. Ryu
中科院分区：
文献类型：
--
作者：
Sungyeun Yang;T. Hwang;Lihua Che;J. Oh;Yoon Ha;W. Ryu
静电纺丝丝素蛋白（SF）支架因其与细胞外基质等天然组织结构相似，且具有良好的生物相容性，可作为替代胶原蛋白的候选材料而备受关注。然而，静电纺丝支架缺乏骨组织支架的物理完整性，骨组织支架需要抵抗机械载荷。在这项工作中，我们提出了膜增强的静电纺丝SF支架通过一系列的过程中，静电纺丝和冷冻干燥的SF解决方案，在两个不同的溶剂：甲酸和水，分别。在湿静电纺丝之后用水代替甲醇，将分散在水中的SF纳米纤维与SF水溶液混合。将混合溶液冷冻干燥得到厚度为几厘米的3D膜连接的SF纳米纤维支架（SF支架）。我们证明了SF水溶液的SF浓度控制纳米纤维之间的膜增强的程度。它还表明，增加膜的增强程度和包括羟基磷灰石（HAP）纳米粒子导致更高的抗压缩负荷的SF支架。人成骨细胞在胶原、SF和SF-HAP支架上的培养结果表明，SF和SF-HAP支架的生物相容性和细胞增殖能力均上级胶原支架。将含有和不含BMP-2的SF-HAP支架用于体内研究4周和8周，并且它们在大鼠颅骨缺损模型中显示出增强的骨组织形成。
Electrospun silk fibroin (SF) scaffolds have drawn much attention because of their resemblance to natural tissue architecture such as extracellular matrix, and the biocompatibility of SF as a candidate material to replace collagen. However, electrospun scaffolds lack the physical integrity of bone tissue scaffolds, which require resistance to mechanical loadings. In this work, we propose membrane-reinforced electrospun SF scaffolds by a serial process of electrospinning and freeze-drying of SF solutions in two different solvents: formic acid and water, respectively. After wet electrospinning followed by replacement of methanol with water, SF nanofibers dispersed in water were mixed with aqueous SF solution. Freeze-drying of the mixed solution resulted in 3D membrane-connected SF nanofibrous scaffolds (SF scaffolds) with a thickness of a few centimeters. We demonstrated that the SF concentration of aqueous SF solution controlled the degree of membrane reinforcement between nanofibers. It was also shown that both increase in degree of membrane reinforcement and inclusion of hydroxyapatite (HAP) nanoparticles resulted in higher resistance to compressive loadings of the SF scaffolds. Culture of human osteoblasts on collagen, SF, and SF-HAP scaffolds showed that both SF and SF-HAP scaffolds had biocompatibility and cell proliferation superior to that of the collagen scaffolds. SF-HAP scaffolds with and without BMP-2 were used for in vivo studies for 4 and 8 weeks, and they showed enhanced bone tissue formation in rat calvarial defect models.