Lipid Extraction and Analysis.

Lipid Extraction and Analysis.
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脂质提取和分析。

DOI:
10.1007/978-1-0716-2181-3_14
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发表时间:
2022
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Watts,JenniferL
Watts,JenniferL
中科院分区:
--
文献类型:
--
作者:
Harrison,HenryH;Watts,JenniferL

文献摘要

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脂质是细胞膜和能量储存的主要成分。脂质有助于重要的结构,能量和信号功能。在标准方法的基础上,优化了秀丽隐杆线虫脂类的提取和分析方法.本文介绍了一种从C. elegans幼虫,成虫,或胚胎。我们描述了一种薄层色谱法分离主要脂质类和气相色谱法分析脂肪酸组成的脂质提取物,脂质馏分,或直接从线虫幼虫,成人,或胚胎。
Lipids are major components of cellular membranes and energy stores. Lipids contribute vital structural, energetic, and signaling functions. We have optimized methods to extract and analyze lipids from the nematodeCaenorhabditis elegansbased on standard methods. Here we describe a method to extract total lipids fromC. eleganslarvae, adults, or embryos. We describe a thin-layer chromatography method to separate major lipid classes and a gas chromatography method to analyze fatty acid composition from lipid extracts, lipid fractions, or directly from nematode larvae, adults, or embryos.