Lipid Extraction and Analysis.
Lipid Extraction and Analysis.
复制标题
脂质提取和分析。
DOI:
10.1007/978-1-0716-2181-3_14
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发表时间:
2022
期刊:
影响因子:
--
通讯作者:
Watts,JenniferL
中科院分区:
文献类型:
--
作者:
Harrison,HenryH;Watts,JenniferL
Lipids are major components of cellular membranes and energy stores. Lipids contribute vital structural, energetic, and signaling functions. We have optimized methods to extract and analyze lipids from the nematodeCaenorhabditis elegansbased on standard methods. Here we describe a method to extract total lipids fromC. eleganslarvae, adults, or embryos. We describe a thin-layer chromatography method to separate major lipid classes and a gas chromatography method to analyze fatty acid composition from lipid extracts, lipid fractions, or directly from nematode larvae, adults, or embryos.