Cytokine production in acute versus chronic human schistosomiasis mansoni:: The cross-regulatory role of interferon-γ and interleukin-10 in the responses of peripheral blood mononuclear cells and splenocytes to parasite antigens
Cytokine production in acute versus chronic human schistosomiasis mansoni:: The cross-regulatory role of interferon-γ and interleukin-10 in the responses of peripheral blood mononuclear cells and splenocytes to parasite antigens
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DOI：
10.1086/314748
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发表时间：
1999-06-01
影响因子：
6.4
通讯作者：
Wynn, TA
中科院分区：
文献类型：
--
作者：
Montenegro, SML;Miranda, P;Wynn, TA
白细胞介素（IL）-10和干扰素（IFN）-γ的1型和2型细胞因子反应的调节的贡献进行了研究，在巴西人与不同的临床形式的曼氏血吸虫病。来自急性肠道血吸虫病家族成员的细胞对可溶性虫卵抗原（SEA）或可溶性成虫抗原制剂（SWAP）的反应比来自几名慢性肠道血吸虫病患者的细胞具有更大量的IFN-γ; IL-10水平相似，IL-10的中和对急性感染患者中SEA特异性IFN-γ应答没有影响，而SWAP诱导的IFN-γ在两组中均增加。抗IL-10还上调了大多数肝脾血吸虫病患者脾细胞培养物中SEA特异性IFN-γ蛋白和mRNA的反应，但对抗原特异性IL-4或IL-5的产生没有影响。IFN-γ的中和导致SWAP特异性IL-10和IL-5的相当的增加，而IL-4不受影响。这些研究表明，血吸虫病的早期疾病与显著的IFN-γ应答相关，并且IL-10有助于在早期和慢性感染期间抑制该应答。
The contribution of interleukin (IL)-10 and interferon (IFN)-gamma to the regulation of type 1 and type 2 cytokine responses was investigated in Brazilians with different clinical forms of schistosomiasis mansoni. Cells from members of a family with acute intestinal schistosomiasis responded to schistosomal soluble egg antigen (SEA) or soluble adult worm antigen preparation (SWAP) with greater amounts of IFN-gamma than did cells from several patients with chronic intestinal schistosomiasis; IL-10 levels were similar, Neutralization of IL-10 had no effect on the SEA-specific IFN-gamma response in patients with acute infection, whereas SWAP-induced IFN-gamma was increased in both groups. Anti-IL-10 also up-regulated SEA-specific IFN-gamma protein and mRNA responses in most splenocyte cultures from hepatosplenic schistosomiasis patients but had no effect on antigen-specific IL-4 or IL-5 production. Neutralization of IFN-gamma resulted in a comparable increase in SWAP-specific IL-10 and IL-5, while IL-4 was not affected. These studies demonstrate that early disease in schistosomiasis is associated with a significant IFN-gamma response and that IL-10 contributes to the suppression of that response during both early and chronic infection.